Department of Orthopaedic Surgery, Jiangyin Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi, China.
Bioengineered. 2022 May;13(5):13849-13859. doi: 10.1080/21655979.2022.2084495.
One of the main causes of low back pain (LBP) and degenerative musculoskeletal disorders is intervertebral disc degeneration (IVDD). Inflammation-associated senescence of Human nucleus pulposus cells (HNPCs) plays an essential function in the disease progression of IVDD. Omentin-1 is an adipokine that has been recently reported to have anti-inflammatory potential. In our research, IL-1β was used to simulate the inflammatory environment in the IVDD. We investigated in vitro the effects of Omentin-1 on HNPCs, including the components of senescence, cell cycle and extracellular matrix (ECM) synthesis. The results showed that the addition of Omentin-1 improved IL-1β-induced senescence in HNPCs. G1 phase cell cycle arrest and reduced ECM synthesis in HNPCs. Furthermore, we demonstrated that the effect of Omentin-1 in reducing senescence of HNPCs is dependent on SIRT1. These findings suggest that Omentin-1 plays an important function in protecting HNPCs against senescence and has the potential for IVDD gene target therapy.
椎间盘退变（IVDD）是导致下腰痛（LBP）和退行性肌肉骨骼疾病的主要原因之一。人髓核细胞（HNPCs）与炎症相关的衰老在 IVDD 的疾病进展中起着重要作用。网膜素-1 是一种脂肪因子，最近有研究报道其具有抗炎潜力。在我们的研究中，使用 IL-1β 模拟 IVDD 中的炎症环境。我们在体外研究了网膜素-1 对 HNPCs 的影响，包括衰老、细胞周期和细胞外基质（ECM）合成的成分。结果表明，添加网膜素-1 可改善 HNPCs 中由 IL-1β 引起的衰老。G1 期细胞周期停滞和 ECM 合成减少。此外，我们证明网膜素-1 降低 HNPCs 衰老的作用依赖于 SIRT1。这些发现表明，网膜素-1 在保护 HNPCs 免受衰老方面起着重要作用，并且有可能成为 IVDD 的基因治疗靶点。
