Department of Spine Surgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu 230003, China; School of Medicine, Southeast University, Nanjing, Jiangsu 230003, China.
Department of Spine Surgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu 230003, China.
Arch Gerontol Geriatr. 2022 Sep-Oct;102:104748. doi: 10.1016/j.archger.2022.104748. Epub 2022 Jun 9.
Intervertebral disc degeneration is an abnormal, cell-mediated process of tissue remodeling, recognized as the principal cause of low back pain affecting 80% of the population worldwide. Inflammatory cytokine, Interleukin-1beta (IL-1β) is involved in the intervertebral disc degeneration (IDD) process, and it is upregulated in degenerated discs. Omentin-1, also known as intelectin-1, is an adipokine with anti-inflammatory, anti-apoptosis, pro-proliferation, and proangiogenic properties in various types of cells. However, little is known about the effects of omentin-1 on human nucleus pulposus cells (HNPCs). This study aims to investigate the effects of omentin-1 on healthy HNPCs regarding proliferation and further investigate the effects of omentin-1 on IL-1β-induced inflammation, apoptosis, and degeneration in HNPCs.
Genes and proteins of interest were measured by qRT-PCR, immunoblotting, and immunofluorescence to conduct related experiments. Cell viability (CCK-8), EdU, and mitochondrial membrane potential (JC-1), flow cytometry assays were used to assess proliferation and apoptosis, respectively.
Our study showed that omentin-1 promoted proliferation in normal HNPCs. Furthermore, omentin-1 expression was decreased in IL-1β-treated HNPCs. Omentin-1 protected against IL-1β-induced inflammation, apoptosis, and degeneration in HNPCs in vitro via the activation of the PI3K/Akt signaling pathway.
These findings may contribute to understanding the role of omentin-1 in HNPCs and may be a potential therapeutic candidate for intervertebral disc degeneration.
椎间盘退变是一种异常的、由细胞介导的组织重塑过程，被认为是导致全球 80%人口出现下腰痛的主要原因。炎性细胞因子白细胞介素-1β（IL-1β）参与椎间盘退变（IDD）过程，在退变的椎间盘中上调。网膜素-1，也称为内特林-1，是一种脂肪因子，具有抗炎、抗凋亡、促增殖和促血管生成作用，在各种类型的细胞中。然而，关于网膜素-1对人髓核细胞（HNPCs）的影响知之甚少。本研究旨在探讨网膜素-1对健康 HNPCs 增殖的影响，并进一步探讨网膜素-1对 HNPCs 中 IL-1β诱导的炎症、凋亡和退变的影响。
通过 qRT-PCR、免疫印迹和免疫荧光测定来测量感兴趣的基因和蛋白质，以进行相关实验。细胞活力（CCK-8）、EdU 和线粒体膜电位（JC-1）、流式细胞术分别用于评估增殖和凋亡。
我们的研究表明，网膜素-1促进了正常 HNPCs 的增殖。此外，在 IL-1β处理的 HNPCs 中，网膜素-1的表达降低。网膜素-1通过激活 PI3K/Akt 信号通路，在体外保护 HNPCs 免受 IL-1β诱导的炎症、凋亡和退变。
这些发现可能有助于理解网膜素-1在 HNPCs 中的作用，并可能成为椎间盘退变的潜在治疗候选物。
