Department of stomatology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Henan University People's Hospital, Zhengzhou, Henan, China.
J Biochem Mol Toxicol. 2022 Aug;36(8):e23118. doi: 10.1002/jbt.23118. Epub 2022 Jun 16.
Circular RNA (circRNA) has been widely reported to be involved in oral squamous cell carcinoma (OSCC), while the way in which hsa_circ_0096042 affects OSCC remains unclear. The hsa_circ_0096042, miR-1288-3p, and KRT4 expression in OSCC tissues and cell lines were detected by quantitative reverse-transcription polymerase chain reaction. Based on the overexpression of hsa_circ_0096042, miR-1288-3p, or KRT4, the viability and proliferation of OSCC cells were analyzed by cell counting kit-8 and colony formation assay, and the protein levels of Bax and Bcl-2 were detected by western blot, and the growth of cancer cells in vivo was analyzed by xenograft experiment. In addition, the database was used to predict the binding of hsa_circ_0096042, miR-1288-3p, and KRT4, and the interaction was confirmed by luciferase, RIP, and RNA pull-down assay. Hsa_circ_0096042 and KRT4 were abnormally downregulated and miR-1288-3p was upregulated in OSCC. Hsa_circ_0096042 overexpression restrained the proliferation and viability of OSCC cells, facilitated apoptosis, and inhibited the growth of cancer cells in vivo. Hsa_circ_0096042 bound to miR-1288-3p, whose upregulation promoted OSCC progression and eliminated the effects of overexpression of hsa_circ_0096042 on OSCC cells. KRT4 was the target gene for miR-1288-3p. Hsa_circ_0096042 plays an antitumor role in OSCC via miR-1288-3p/KRT4 axis.
环状 RNA (circRNA) 已被广泛报道参与口腔鳞状细胞癌 (OSCC)，但其影响 OSCC 的方式尚不清楚。通过定量逆转录聚合酶链反应检测 OSCC 组织和细胞系中的 hsa_circ_0096042、miR-1288-3p 和 KRT4 的表达。基于 hsa_circ_0096042、miR-1288-3p 或 KRT4 的过表达，通过细胞计数试剂盒-8 和集落形成测定分析 OSCC 细胞的活力和增殖，通过 Western blot 检测 Bax 和 Bcl-2 的蛋白水平，并通过异种移植实验分析癌细胞的体内生长情况。此外，使用数据库预测 hsa_circ_0096042、miR-1288-3p 和 KRT4 的结合，并通过荧光素酶、RIP 和 RNA 下拉实验验证相互作用。hsa_circ_0096042 和 KRT4 在 OSCC 中异常下调，miR-1288-3p 上调。hsa_circ_0096042 过表达抑制 OSCC 细胞的增殖和活力，促进细胞凋亡，并抑制癌细胞在体内的生长。hsa_circ_0096042 与 miR-1288-3p 结合，其上调促进 OSCC 进展并消除 hsa_circ_0096042 对 OSCC 细胞的影响。KRT4 是 miR-1288-3p 的靶基因。hsa_circ_0096042 通过 miR-1288-3p/KRT4 轴在 OSCC 中发挥抗肿瘤作用。
