芦笋内生真菌 Phomopsis asparagi LSLYZ-87 来源的吡喃酮衍生物。

Pyrone Derivatives from a Mangrove Endophytic Fungus Phomopsis asparagi LSLYZ-87.

机构信息

College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health, Research Institute for Marine Drugs and Nutrition, Guangdong Ocean University, Zhanjiang, 524088, P. R. China.

School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, P. R. China.

出版信息

Chem Biodivers. 2022 Aug;19(8):e202200491. doi: 10.1002/cbdv.202200491. Epub 2022 Jun 23.

DOI:10.1002/cbdv.202200491

PMID:35707944

Abstract

Chemical investigation of the endophytic fungus Phomopsis asparagi LSLYZ-87 cultured on PDB medium led to the isolation of two new pyrone derivatives, phomasparapyrone A (1), and phomasparapyrone B (2), together with the known kojic acid (3). Their planar structures were connected through 1D and 2D NMR spectroscopic data. And the stereo structures of 1 and 2 were defined by comparison of the experimental ECD spectra to calculated one. All isolates were evaluated for their anti-neuroinflammatory activities. Among them, compound 2 showed moderate inhibition on NO accumulation induced by LPS on BV-2 cells in a dose dependent manner at 30, 40 and 50 μM, and without cytotoxicity in a concentration of 50.0 μM.

摘要

从 PDB 培养基中培养的内生真菌 Phomopsis asparagi LSLYZ-87 的化学成分研究中，分离得到了两个新的吡喃酮衍生物，即 phomasparapyrone A（1）和 phomasparapyrone B（2），以及已知的曲酸（3）。通过 1D 和 2D NMR 光谱数据连接它们的平面结构。通过将实验 ECD 光谱与计算光谱进行比较，确定了 1 和 2 的立体结构。所有分离物均评估了其抗神经炎症活性。其中，化合物 2 在 30、40 和 50μM 时，以剂量依赖的方式对 LPS 诱导的 BV-2 细胞中 NO 积累表现出适度的抑制作用，在 50.0μM 浓度下没有细胞毒性。