Silprasert A, Dejsarai W, Keawvichit R, Amatayakul K
Hum Nutr Clin Nutr. 1987 Jan;41(1):31-6.
Human milk samples were collected from mothers on the third post-partum day with a manual breast-pump and pooled. They were divided into aliquots and stored at room temperature (24-25 degrees C), and at 4 degrees, 37 degrees and -20 degrees C. The creamatocrit (CR) values decreased significantly after storage at room temperature (P less than 0.03), or at 37 degrees C (P less than 0.001) for 45 min and 30 min, respectively. Creamatocrit remained stable for 14 d at 4 degrees C, and for up to 28 d at -20 degrees C. However, freezing and thawing samples twice significantly decreased creamatocrit values (P less than 0.001). The total energy concentration of the samples stored at -20 degrees C was stable for 28 d. These results indicate that strict precautions must be taken when handling and storing human milk samples for analysis of lipid content.
在产后第三天，用手动吸奶器从母亲那里收集母乳样本并混合。将它们分成小份，分别储存在室温（24 - 25摄氏度）、4摄氏度、37摄氏度和 -20摄氏度下。在室温下储存45分钟（P < 0.03）或在37摄氏度下储存30分钟（P < 0.001）后，乳脂率（CR）值显著下降。乳脂率在4摄氏度下可保持14天稳定，在 -20摄氏度下可保持长达28天稳定。然而，样本冻融两次会显著降低乳脂率值（P < 0.001）。储存在 -20摄氏度下的样本总能量浓度在28天内保持稳定。这些结果表明，在处理和储存用于脂质含量分析的母乳样本时必须采取严格的预防措施。
