Vázquez-Román S, Alonso-Díaz C, García-Lara N R, Escuder-Vieco D, Pallás-Alonso C R
Servicio de Neonatología, Hospital Universitario Doce de Octubre, Madrid, España.
Servicio de Neonatología, Hospital Universitario Doce de Octubre, Madrid, España.
An Pediatr (Barc). 2014 Sep;81(3):185-8. doi: 10.1016/j.anpedi.2013.09.001. Epub 2013 Dec 4.
To determine, by the creamatocrit measurement, the effect on the fat content of raw and pasteurized donor milk of freezing during 3 months at -20 °C.
The evolution of the creamatocrit measurement (following Lucas technique) on frozen (-20 °C), raw and pasteurized human milk, was analyzed during 3 months.
The fat content of raw milk (n=44) was 3.19 g/dl at the beginning and 2.86 g/dl after 3 months frozen (p=0.02). In pasteurized milk (n=36) fat content at the first determination was 2.59 g/dl and 2.20 g/dl after 1 month frozen (p=0.01). Afterwards there were no significant changes up to 3 months frozen. Variability was observed in the intermediate values.
A reduction on the fat content measurement of raw and pasteurized donor human milk after freezing was observed. Freezing does not inactivate the milk lipase but does destroy the fat globule. Creamatocrit measurement may not be the best method to determine the fat content of processed human milk.
通过测定乳脂比容，确定在-20°C下冷冻3个月对生鲜和巴氏杀菌供体母乳脂肪含量的影响。
分析了冷冻（-20°C）的生鲜和巴氏杀菌人乳在3个月内乳脂比容（采用卢卡斯技术）的变化情况。
生鲜乳（n = 44）开始时脂肪含量为3.19 g/dl，冷冻3个月后为2.86 g/dl（p = 0.02）。巴氏杀菌乳（n = 36）首次测定时脂肪含量为2.59 g/dl，冷冻1个月后为2.20 g/dl（p = 0.01）。此后，直至冷冻3个月都没有显著变化。中间值存在变异性。
观察到冷冻后生鲜和巴氏杀菌供体人乳的脂肪含量测定值降低。冷冻不会使乳脂肪酶失活，但会破坏脂肪球。乳脂比容测定可能不是确定加工后人乳脂肪含量的最佳方法。
