Vergara J A, Raymond U, Thet L A
Int J Radiat Oncol Biol Phys. 1987 May;13(5):723-32. doi: 10.1016/0360-3016(87)90291-4.
We used stereologic-morphometric techniques to obtain a detailed quantitative picture of the changes in lung ultrastructure of rats at 12 and 26 weeks after unilateral thoracic irradiation with 3000 cGy. At 12 weeks post-radiation, the total number type 1 epithelial cells, type 2 epithelial cells and capillary endothelial cells were decreased 50-70%, total type 1 epithelial and capillary surface areas were decreased 55-60%, and the total volume of intracapillary blood was decreased 75%. The interstitial cells and matrix together accounted for more than 9% of the peripheral lung tissue volume including air, compared to 3% in controls. The numerical density of interstitial cells was increased to 3-fold the control value. The numerical density of interstitial cells was increased to 3-fold the control value. Although fibroblasts still comprised the largest interstitial cell subgroup, the numerical density of mast cells was increased over 150-fold and other inflammatory and immune cells were increased to a lesser extent. At 26 weeks post-radiation, the number, volume, and surface area of the type 1 epithelium and capillary endothelium had further decreased to only 5-10% of control values. The total number of type 2 epithelial cells was reduced by 75% but the volume density was actually increased because of a 4-fold increase in the mean cell volume. The interstitial cells and matrix now comprised over 77% of total peripheral lung tissue volume including air as compared to 6% in controls. Mast cells and plasma cells comprised 11% and 19% of all interstitial cells respectively and the densities of these cells were 540 and 180-fold the control value respectively. The relation of these morphometric findings to the results of previous morphologic studies is discussed.
我们采用体视学-形态测量技术，对接受3000厘戈瑞单侧胸部照射的大鼠在照射后12周和26周时肺超微结构的变化进行了详细的定量描述。照射后12周，I型上皮细胞、II型上皮细胞和毛细血管内皮细胞的总数减少了50%-70%，I型上皮细胞和毛细血管的总面积减少了55%-60%，毛细血管内血液的总体积减少了75%。间质细胞和基质在包括空气的外周肺组织体积中所占比例超过9%，而对照组为3%。间质细胞的数量密度增加到对照值的3倍。尽管成纤维细胞仍然是间质细胞的最大亚群，但肥大细胞的数量密度增加了150倍以上，其他炎性和免疫细胞也有不同程度的增加。照射后26周，I型上皮细胞和毛细血管内皮细胞的数量、体积和表面积进一步减少至对照值的5%-10%。II型上皮细胞的总数减少了75%，但其体积密度实际上增加了，因为平均细胞体积增加了4倍。间质细胞和基质现在在包括空气的外周肺组织总体积中所占比例超过77%，而对照组为6%。肥大细胞和浆细胞分别占所有间质细胞的11%和19%，这些细胞的密度分别是对照值的540倍和180倍。本文讨论了这些形态测量结果与以往形态学研究结果的关系。
