Department of Dermatology, CHUV-FBM UNIL, Beaumont Hospital, Lausanne, Switzerland.
University of Lausanne, Lausanne, Switzerland.
Exp Dermatol. 2022 Aug;31(8):1276-1280. doi: 10.1111/exd.14626. Epub 2022 Jun 23.
Primary cilium (PC) is a microtubule-based organelle found on the apical surface of most mammalian cell types, playing a role in development and tissue homeostasis. Ciliopathies are a rapidly growing group of human diseases characterized by disordered cilium. PC plays an important role in pathogenesis of basal cell cancer, the most common human malignancy. A significant increase in ciliation has been observed in the epidermis of atopic dermatitis and psoriasis patients. Spontaneously immortalized human keratinocytes, HaCaT are a model to study the epidermal homeostasis and pathophysiology. In contrast to what has been previously described, here, we show that HaCaT can be efficiently ciliated. In HaCaT cells, differentiation significantly increased the number of ciliated cells and we were able to analyse in detail the ciliary length progression with duration of differentiation. As the number of recognized ciliopathies continues to increase, the importance of ciliary models also rises. Even though keratinocytes do not become as highly and rapidly ciliated as cell lines frequently used in ciliary studies, they are a better model for the study of skin ciliopathies. Detailed progression of ciliation in HaCaT could serve as the basis for ciliary studies in this cell line.
初级纤毛（PC）是一种存在于大多数哺乳动物细胞顶表面的微管细胞器，在发育和组织稳态中发挥作用。纤毛病是一组快速增长的人类疾病，其特征是纤毛紊乱。PC 在基底细胞癌的发病机制中起重要作用，基底细胞癌是最常见的人类恶性肿瘤。特应性皮炎和银屑病患者的表皮中观察到纤毛数量显著增加。自发永生化的人角质形成细胞 HaCaT 是研究表皮稳态和病理生理学的模型。与之前描述的情况相反，我们在这里显示 HaCaT 可以被有效地纤毛化。在 HaCaT 细胞中，分化显著增加了纤毛细胞的数量，并且我们能够详细分析随着分化时间的延长，纤毛长度的进展。随着被识别的纤毛病数量不断增加，纤毛模型的重要性也在增加。尽管角质形成细胞不像纤毛研究中常用的细胞系那样高度和快速地纤毛化，但它们是研究皮肤纤毛病的更好模型。HaCaT 中的纤毛发生详细进展可以作为该细胞系中纤毛研究的基础。
