Tabakhiyan Fahimeh, Mir Amirabbas, Vahedian Vahid
Department of Biology, Ahar Branch, Islamic Azad University, Ahar, Iran.
Institute of Nano Science and Nano Technology, University of Kashan, Kashan, Islamic Republic of Iran.
Horm Mol Biol Clin Investig. 2022 Jun 16;43(4):389-396. doi: 10.1515/hmbci-2022-0028. eCollection 2022 Dec 1.
Hepatocellular carcinoma (HCC), the most common form of liver cancer, is a leading cause of tumor-associated mortality worldwide. Diagnosis based upon non-invasive criteria is currently challenged by the need for molecular information that requires tissue or liquid biopsies. The progression of HCC is often associated with chronic inflammation, expression levels of inflammatory mediators, chemokine, and cytokines. In this study, we try to evaluate the PI3K and pro-inflammatory cytokines, TGF-β, IL-1, and IL-6 expression level in patients with liver cancer.
The kupffer cells were isolated from patient's specimens. Real-time PCR was applied to evaluate the expression level of PI3K in cell lines or tumors. The concentrations of TGF-β, IL-1, and IL-6 were measured by the quantitative ELISA kit.
PI3K mRNA expression in cancer cells was increased markedly vs. normal cells. The ELISA results demonstrated over expression of TGF-β, IL-1, and IL-6 in patients and positive correlation between tumor size and stage.
This study suggests that targeting the expression level of PI3K and pro-inflammatory chemokine and cytokines, TGF-β, IL-1, and IL-6, may be a potential diagnostic strategy in HCC patients.
肝细胞癌（HCC）是最常见的肝癌形式，是全球肿瘤相关死亡的主要原因。基于非侵入性标准的诊断目前面临挑战，因为需要通过组织活检或液体活检获取分子信息。HCC的进展通常与慢性炎症、炎症介质、趋化因子和细胞因子的表达水平相关。在本研究中，我们试图评估肝癌患者中PI3K和促炎细胞因子转化生长因子-β（TGF-β）、白细胞介素-1（IL-1）和白细胞介素-6（IL-6）的表达水平。
从患者标本中分离枯否细胞。应用实时聚合酶链反应（PCR）评估细胞系或肿瘤中PI3K的表达水平。采用定量酶联免疫吸附测定（ELISA）试剂盒检测TGF-β、IL-1和IL-6的浓度。
与正常细胞相比，癌细胞中PI3K信使核糖核酸（mRNA）表达显著增加。ELISA结果显示患者中TGF-β、IL-1和IL-6表达上调，且肿瘤大小与分期呈正相关。
本研究表明，针对PI3K以及促炎趋化因子和细胞因子TGF-β、IL-1和IL-6的表达水平进行干预，可能是HCC患者一种潜在的诊断策略。
