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哺乳动物食管复层鳞状上皮中干细胞的鉴定与特性分析

Identification and characterization of stem cells in mammalian esophageal stratified squamous epithelia.

作者信息

Yang Yanan, Deng Guodong, Qiao Lili, Yuan Hui, Yu Xiaohong, Xu Lei, Lu Shih-Hsin, Jiang Wei, Yu Xiying

机构信息

Department of Etiology and Carcinogenesis, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

出版信息

J Mol Cell Biol. 2022 Nov 17;14(6). doi: 10.1093/jmcb/mjac038.

DOI:10.1093/jmcb/mjac038
PMID:35709398
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9669669/
Abstract

Somatic stem cells are essential for the maintenance of tissue homeostasis. Despite its importance, how the esophageal stratified squamous epithelium executes its self-renewal and maintenance remains elusive. In this study, using 5-bromo-2'-deoxyuridine label-chase in rats in vivo and rat esophageal organoids in vitro together with genome-wide DNA methylation and single-cell RNA sequencing, we identified a slow-cycling/quiescent stem cell population that contained high levels of hemidesmosomes (HDs) and low levels of Wnt signaling localized spatially and randomly at the basal layer of the esophageal epithelium. Pseudotime cell trajectory analysis indicated that tissue cells originated from quiescent basal stem cells in the basal layer. Perturbations of HD component expression and/or Wnt signaling reduced the stem cell population in the basal layer of esophageal keratinocyte organoids, resulting in alterations in the organoid formation rate, size, morphogenesis, and proliferation-differentiation homeostasis. Furthermore, not only high levels of HDs and low levels of Wnt signaling but also an interplay between HD and Wnt signaling defined the stem cells of the basal layer. Hence, HDs and Wnt signaling are critical determinants for defining the stem cells of the basal layer required for tissue homeostasis in mammalian esophagi.

摘要

体细胞干细胞对于维持组织稳态至关重要。尽管其重要性，但食管复层鳞状上皮如何进行自我更新和维持仍不清楚。在本研究中，我们在体内对大鼠使用5-溴-2'-脱氧尿苷标记追踪，并结合全基因组DNA甲基化和单细胞RNA测序，在体外培养大鼠食管类器官，从而鉴定出一个慢循环/静止干细胞群体，该群体在食管上皮基底层中空间上随机分布，含有高水平的半桥粒（HDs）和低水平的Wnt信号。伪时间细胞轨迹分析表明，组织细胞起源于基底层的静止基底干细胞。HD成分表达和/或Wnt信号的扰动减少了食管角质形成细胞类器官基底层中的干细胞群体，导致类器官形成率、大小、形态发生以及增殖-分化稳态发生改变。此外，不仅高水平的HDs和低水平的Wnt信号，而且HD和Wnt信号之间的相互作用也定义了基底层的干细胞。因此，HDs和Wnt信号是定义哺乳动物食管组织稳态所需基底层干细胞的关键决定因素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/ec7c0dee6e06/mjac038fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/c7905d8c1af3/mjac038fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/2b145a08cbe7/mjac038fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/51d06622b1d9/mjac038fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/cfc805b2c7e6/mjac038fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/07c4e7f628f2/mjac038fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/3be3eee7ac90/mjac038fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/3be3eee7ac90/mjac038fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/ec7c0dee6e06/mjac038fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/c7905d8c1af3/mjac038fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/2b145a08cbe7/mjac038fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/51d06622b1d9/mjac038fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/cfc805b2c7e6/mjac038fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/07c4e7f628f2/mjac038fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/3be3eee7ac90/mjac038fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/3be3eee7ac90/mjac038fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bd5/9669669/ec7c0dee6e06/mjac038fig7.jpg

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2
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Development. 2021 Mar 29;148(6):dev193839. doi: 10.1242/dev.193839.
3
Human gastrointestinal epithelia of the esophagus, stomach, and duodenum resolved at single-cell resolution.人类食管、胃和十二指肠的胃肠道上皮以单细胞分辨率得到解析。
人乳头瘤病毒E6如何操纵宿主细胞分化过程以维持感染库。
Tumour Virus Res. 2025 Jan 19;19:200313. doi: 10.1016/j.tvr.2025.200313.
4
Deep learning enables stochastic optical reconstruction microscopy-like superresolution image reconstruction from conventional microscopy.深度学习能够从传统显微镜图像中实现类似随机光学重建显微镜的超分辨率图像重建。
iScience. 2023 Oct 4;26(11):108145. doi: 10.1016/j.isci.2023.108145. eCollection 2023 Nov 17.
5
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Cell Rep. 2021 Mar 9;34(10):108819. doi: 10.1016/j.celrep.2021.108819.
4
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5
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