Orekhov A N, Krushinsky A V, Andreeva E R, Repin V S, Smirnov V N
Heart Vessels. 1986;2(4):193-201. doi: 10.1007/BF02059968.
Adult human aortic cells have different shapes in situ. To determine whether populations of cultured aortic cells are also polymorphic, a technique for separation of cells from the intimal and medial layers of the human aorta by enzymatic dispersion of the vascular tissue was employed. It was established that aortic cells are polymorphic in primary culture, at least within the first 7 days after seeding. Four main morphological cell types were identified--elongated, asymmetric, polygonal, and stellate. Polygonal and stellate cells are found only in cultures of grossly normal intima. Elongated and asymmetric cells are present in practically all cultures. The ratio of elongated to asymmetric cells in cultures obtained from healthy aortas and atherosclerotic plaques is more or less the same and is approximately 3:1. In cultures of fatty streaks, the portion of asymmetric cells exceeds 50%. With immunofluorescent staining and ultra-structural analysis, cells of all four types were identified as smooth muscle. Possible reasons for the cells polymorphism in primary culture and the prospects of utilizing this culture method in the investigation of cellular aspects of atherogenesis are discussed.
成年人类主动脉细胞在原位具有不同的形态。为了确定培养的主动脉细胞群体是否也具有多态性，采用了一种通过酶分散血管组织从人主动脉内膜和中膜层分离细胞的技术。已确定主动脉细胞在原代培养中具有多态性，至少在接种后的前7天内如此。鉴定出四种主要的形态学细胞类型——细长型、不对称型、多边形和星状。多边形和星状细胞仅在大体正常内膜的培养物中发现。细长型和不对称型细胞几乎存在于所有培养物中。从健康主动脉和动脉粥样硬化斑块获得的培养物中，细长型细胞与不对称型细胞的比例大致相同，约为3:1。在脂肪条纹的培养物中，不对称型细胞的比例超过50%。通过免疫荧光染色和超微结构分析，所有四种类型的细胞均被鉴定为平滑肌细胞。讨论了原代培养中细胞多态性的可能原因以及利用这种培养方法研究动脉粥样硬化发生的细胞方面的前景。
