Mount M E, Failla D L
J Assoc Off Anal Chem. 1987 Mar-Apr;70(2):201-5.
Monensin is converted to monensin bromoacetate, which provides successful coupling to protein and allows production of monensin-specific rabbit antisera. A modified indirect enzyme-linked immunosorbent assay (ELISA) was developed, which is highly sensitive (2 ng/mL) to monensin determination. Monensin recovery was 53-81% at 10 ppb in sera or urine, and 81-130% at 100 ppb in dichloromethane-extracted feces or water. Overall recovery was 98.8% with coefficients of variation from 6 to 52% over the range of monensin concentrations studied. This is the first immunoassay reported for the carboxylic ionophore antibiotics.
莫能菌素被转化为莫能菌素溴乙酸酯，它能成功与蛋白质偶联，并可用于制备莫能菌素特异性兔抗血清。开发了一种改良的间接酶联免疫吸附测定法（ELISA），该方法对莫能菌素的测定具有高度敏感性（2 ng/mL）。在血清或尿液中，10 ppb时莫能菌素的回收率为53 - 81%，在二氯甲烷萃取的粪便或水中，100 ppb时回收率为81 - 130%。在所研究的莫能菌素浓度范围内，总体回收率为98.8%，变异系数为6%至52%。这是首次报道的针对羧酸离子载体抗生素的免疫测定法。
