Rittenburg J H, Adams A, Palmer J, Allen J C
J Assoc Off Anal Chem. 1987 May-Jun;70(3):582-7.
An indirect, competitive enzyme-linked immunosorbent assay (ELISA) has been developed for quantitation of soy protein in meat products. The methodology allows rapid aqueous extraction of meat samples into a liquid form suitable for assay. The assay is highly specific for soy protein and is designed to measure soy protein levels between 1 and 10% of the wet weight of the sample. Standardized, stabilized reagents for carrying out the procedure are commercially available in a kit. The analysis, including sample preparation, can be completed within a workday, and the actual immunoassay in less than 60 min.
已开发出一种间接竞争酶联免疫吸附测定法（ELISA），用于定量肉制品中的大豆蛋白。该方法可将肉类样品快速水提取成适合检测的液体形式。该测定法对大豆蛋白具有高度特异性，旨在测量样品湿重的1%至10%之间的大豆蛋白水平。用于执行该程序的标准化、稳定化试剂可在试剂盒中以商业方式获得。包括样品制备在内的分析可在一个工作日内完成，实际免疫测定不到60分钟。
