Teraoka H, Tsukada K
J Biochem. 1987 Jan;101(1):225-31. doi: 10.1093/oxfordjournals.jbchem.a121895.
High concentrations of the nonspecific macromolecule polyethylene glycol 6000 (PEG 6000) enabled DNA ligases I and II from calf thymus to catalyze intermolecular blunt-end ligation of duplex DNA. Intermolecular cohesive-end ligation with these enzymes was markedly stimulated in the presence of 10-16% (w/v) PEG 6000. The effect of PEG 6000 (4-16%) on the sealing of single-stranded breaks in duplex DNA with DNA ligases I and II was not appreciably stimulatory but rather inhibitory. PEG 6000 (15%) enhanced more twofold the rate of DNA ligase II-AMP complex formation, but moderately suppressed the rate of formation of DNA ligase 1-AMP complex. Polyamines and KCl inhibited blunt-end and cohesive-end ligations with DNA ligases I and II in the presence of PEG 6000.
高浓度的非特异性大分子聚乙二醇6000（PEG 6000）能使来自小牛胸腺的DNA连接酶I和II催化双链DNA的分子间平端连接。在10 - 16%（w/v）PEG 6000存在的情况下，这些酶的分子间粘性末端连接受到显著刺激。PEG 6000（4 - 16%）对DNA连接酶I和II封闭双链DNA中单链断裂的作用并非明显具有刺激作用，而是具有抑制作用。PEG 6000（15%）使DNA连接酶II - AMP复合物的形成速率提高了两倍多，但适度抑制了DNA连接酶I - AMP复合物的形成速率。在PEG 6000存在的情况下，多胺和KCl抑制了DNA连接酶I和II的平端和粘性末端连接。
