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聚合物刺激连接：在聚合物溶液中，T4 DNA连接酶增强DNA或脱氧核糖寡核苷酸的平端或粘性末端连接。

Polymer-stimulated ligation: enhanced blunt- or cohesive-end ligation of DNA or deoxyribooligonucleotides by T4 DNA ligase in polymer solutions.

作者信息

Pheiffer B H, Zimmerman S B

出版信息

Nucleic Acids Res. 1983 Nov 25;11(22):7853-71. doi: 10.1093/nar/11.22.7853.

DOI:10.1093/nar/11.22.7853
PMID:6359064
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326544/
Abstract

The rates of blunt-end and cohesive-end ligation of DNA by T4 DNA ligase are increased by orders of magnitude in the presence of high concentrations of a variety of nonspecific polymers such as polyethylene glycol, Ficoll, bovine plasma albumin, or glycogen. Blunt-end ligation of small self-complementary oligodeoxyribonucleotides is also stimulated. The use of polyethylene glycol 6000 in such systems is characterized in some detail. Conditions are suggested which either greatly increase the rate of formation of and size of linear ligation products or which allow smaller but significant improvements in the amounts of circular ligation products.

摘要

在存在高浓度的多种非特异性聚合物（如聚乙二醇、菲可、牛血浆白蛋白或糖原）的情况下，T4 DNA连接酶对DNA平端和粘性末端的连接速率会提高几个数量级。小的自我互补寡脱氧核糖核苷酸的平端连接也会受到刺激。本文详细描述了在这类体系中聚乙二醇6000的使用情况。文中提出了一些条件，这些条件要么能极大地提高线性连接产物的形成速率和大小，要么能使环状连接产物的量有较小但显著的增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/240bc1e2ab25/nar00367-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/a0ac24250d77/nar00367-0204-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/ce446882a158/nar00367-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/306a4acd2f9b/nar00367-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/a420ddbef9d2/nar00367-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/c501ee878819/nar00367-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/b4a72afb23ea/nar00367-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/3cc109c8ea77/nar00367-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/7ff06ad227f1/nar00367-0215-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/240bc1e2ab25/nar00367-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/a0ac24250d77/nar00367-0204-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/ce446882a158/nar00367-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/306a4acd2f9b/nar00367-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/a420ddbef9d2/nar00367-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/c501ee878819/nar00367-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/b4a72afb23ea/nar00367-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/3cc109c8ea77/nar00367-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/7ff06ad227f1/nar00367-0215-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5206/326544/240bc1e2ab25/nar00367-0216-a.jpg

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2
Catenation of DNA rings by topoisomerases. Mechanism of control by spermidine.拓扑异构酶对DNA环的连环化作用。亚精胺的调控机制。
J Biol Chem. 1982 Mar 10;257(5):2687-93.
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Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳测定小双链和单链DNA分子的链长
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BioBrick-based 'Quick Gene Assembly' .基于生物砖的“快速基因组装”
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A single-molecule sequencing assay for the comprehensive profiling of T4 DNA ligase fidelity and bias during DNA end-joining.一种单分子测序分析方法，用于全面分析 T4 DNA 连接酶在 DNA 末端连接过程中的保真度和偏好性。
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