Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, Republic of Korea; Department of Bionanotechnology and Bioconvergence Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, Republic of Korea.
Carbon Nano Convergence Technology Center for Next Generation Engineers (CNN), Jeonbuk National University, Jeonju City 561-756, Republic of Korea; Cellco Inc., Jeonju University, Cheonjam-ro303, Wansan-gu 55069, Republic of Korea.
J Colloid Interface Sci. 2022 Nov;625:12-23. doi: 10.1016/j.jcis.2022.05.020. Epub 2022 May 7.
A novel and facile synthesis is made of cotton-like three-dimensional (3D) fibrous scaffold containing spatiotemporally defined patterns of simvastatin (SIM) optimized for angiogenesis-coupled osteogenesis. Herein, we demonstrate the 3D fiber deposition mechanism in detail during the electrospinning process via computer simulation. The 3D fibrous scaffolds were functionalized with hydroxyapatite nanoparticles (HA - NPs) to induce the biomineralization process mimicking the natural apatite layer. The morphology, physiochemical properties, biomimetic mineralization, and drug release of the as-fabricated 3D fibrous scaffolds of simvastatin-loaded poly (ɛ-caprolactone) poly (glycerol-sebacate) hydroxyapatite nanoparticles (3D - PGHS) were investigated. The effects of simvastatin on the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and angiogenesis in human umbilical vein endothelial cells (HUVECs) were assessed. The results showed that the 3D - PGHS both enhanced the expression of osteogenic markers including ALP, RUNX2, and COLA1 in hMSCs, and promoted the migration and tube formation of HUVECs. This finding demonstrates the potential of 3D scaffold-loaded SIM as a putative point-of-care therapy for tightly controlled tissue regeneration.
一种新颖且简便的方法合成了具有棉状三维（3D）纤维支架，其中包含时空限定的辛伐他汀（SIM）图案，这种图案优化后可促进血管生成耦联的成骨作用。在此，我们通过计算机模拟详细展示了电纺过程中的 3D 纤维沉积机制。3D 纤维支架用纳米羟基磷灰石（HA-NPs）进行功能化，以诱导仿生矿化过程，模拟天然磷灰石层。研究了载有辛伐他汀的聚（ε-己内酯）聚（甘油琥珀酸酯）羟基磷灰石纳米粒子（3D-PGHS）的 3D 纤维支架的形态、物理化学性质、仿生矿化和药物释放。评估了辛伐他汀对人骨髓间充质干细胞（hMSCs）成骨分化和人脐静脉内皮细胞（HUVECs）血管生成的影响。结果表明，3D-PGHS 均增强了 hMSCs 中包括碱性磷酸酶（ALP）、RUNX2 和 COLA1 等成骨标志物的表达，并促进了 HUVECs 的迁移和管腔形成。这一发现表明，载有 SIM 的 3D 支架作为一种潜在的即时治疗方法，可用于严格控制组织再生。
