Li Jialiang, Li Jiaxi, Yang Yubing, He Xijing, Wei Xinyu, Tan Qinghua, Wang Yiqun, Xu Siyue, Chang Sue, Liu Weiwei
Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Department of Health Management, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Open Life Sci. 2023 Jan 24;18(1):20220530. doi: 10.1515/biol-2022-0530. eCollection 2023.
Beta-tricalcium phosphate (β-TCP) bioceramics have an inorganic composition similar to the human bone. While conventional methods can only produce ceramic scaffolds with poor controllability, the advancement of 3D-printing, especially stereolithography, made it possible to manufacture controllable, highly precise, micropore ceramic scaffolds. In this study, the stereolithography was applied to produce β-TCP bioceramics, while ZrO, AlO, Ti6Al4V, and polyetheretherketone (PEEK) were used as controls. Phase analysis, water contact angle tests, and Micro-CT were applied to evaluate the surface properties and scaffold. Hemolytic toxicity, cell proliferation, and morphological assessment were performed to evaluate the biocompatibility. Alkaline phosphatase (ALP) level, mineralization, and qRT-PCR were measured to evaluate the osteointegration. During the manufacturing of β-TCP, no evident impurity substance and hemolytic toxicity was found. Cells on β-TCP had good morphologies, and their proliferation capability was similar to Ti6Al4V, which was higher than the other materials. Cells on β-TCP had higher ALP levels than PEEK. The degree of mineralization was significantly higher on β-TCP. The expression of osteogenesis-related genes on β-TCP was similar to Ti6Al4V and higher than the other materials. In this study, the β-TCP produced by stereolithography had no toxicity, high accuracy, and excellent osteointegration capability, thus resulting as a good choice for bone implants.
β-磷酸三钙（β-TCP）生物陶瓷具有与人体骨骼相似的无机成分。传统方法只能生产可控性差的陶瓷支架，而3D打印技术的进步，尤其是立体光刻技术，使得制造可控、高精度的微孔陶瓷支架成为可能。在本研究中，采用立体光刻技术制备β-TCP生物陶瓷，同时使用ZrO、AlO、Ti6Al4V和聚醚醚酮（PEEK）作为对照。通过相分析、水接触角测试和显微CT来评估表面性能和支架。进行溶血毒性、细胞增殖和形态学评估以评价生物相容性。测量碱性磷酸酶（ALP）水平、矿化程度和qRT-PCR以评估骨整合情况。在制备β-TCP的过程中，未发现明显的杂质物质和溶血毒性。β-TCP上的细胞形态良好，其增殖能力与Ti6Al4V相似，高于其他材料。β-TCP上的细胞ALP水平高于PEEK。β-TCP上的矿化程度明显更高。β-TCP上成骨相关基因的表达与Ti6Al4V相似，高于其他材料。在本研究中，通过立体光刻技术制备的β-TCP无毒、精度高且具有优异的骨整合能力，因此是骨植入物的良好选择。
