The Third Affiliated Hospital of Zhengzhou University, China.
The Third Affiliated Hospital of Zhengzhou University, China.
Reprod Biol. 2022 Sep;22(3):100665. doi: 10.1016/j.repbio.2022.100665. Epub 2022 Jun 14.
To investigate the influences of fresh embryo transfer (ET) and frozen embryo transfer (FET) on neonatal birthweight and the expression of imprinted genes PEG10 and L3MBTL1 in the placenta after in vitro fertilization-embryo transfer (IVF-ET), we analyzed the neonatal birthweight between fresh ET and FET transfer cycles. Then, we collected placentas delivered by fresh ET and FET, and real-time quantitative PCR, Western blotting and immunohistochemistry were used to detect the expression of PEG10 and L3MBTL1. The mean neonatal birthweight of fresh ET was lower than that of FET(3348.48 ± 521.05 vs. 3450.34 ± 524.13, P < 0.001). The risks of low birthweight (LBW) and small-for-gestational age (SGA) were lower after FET (3.9 % vs. 5.4 %; 7.2 % vs. 10.3 %), with adjusted rate ratios of 0.74 (95 % CI, 0.59-0.93; P = 0.003) and 0.70 (95 % CI, 0.59-0.84; P < 0.001), respectively. FET resulted in higher frequencies of macrosomia and large-for-gestational age (LGA) (14.2 % vs. 10.3; 11.0 % vs. 7.1 %) than fresh ET, with adjusted rate ratios of 1.45 (95 % CI, 1.26-1.68; P < 0.001) and 1.62 (95 % CI, 1.37-1.91; P < 0.001), respectively. We also observed PEG10 mRNA and protein expression levels in placentas delivered by fresh ET and FET were significantly different, but there were no significant differences in L3MBTL1 between the two groups. Fresh ET may affect the expression of the imprinted gene PEG10 in the placenta and adverse to placental implantation and development, resulting to increasing incidences of LBW and SGA.
为了研究新鲜胚胎移植（ET）和冷冻胚胎移植（FET）对体外受精-胚胎移植（IVF-ET）后新生儿出生体重和印迹基因 PEG10 和 L3MBTL1 在胎盘表达的影响，我们分析了新鲜 ET 和 FET 移植周期之间的新生儿出生体重。然后，我们收集新鲜 ET 和 FET 分娩的胎盘，并使用实时定量 PCR、Western 印迹和免疫组织化学检测 PEG10 和 L3MBTL1 的表达。新鲜 ET 的新生儿平均出生体重低于 FET（3348.48±521.05 vs. 3450.34±524.13，P<0.001）。FET 后低出生体重（LBW）和小于胎龄儿（SGA）的风险较低（3.9% vs. 5.4%；7.2% vs. 10.3%），调整后的率比分别为 0.74（95%CI，0.59-0.93；P=0.003）和 0.70（95%CI，0.59-0.84；P<0.001）。FET 后巨大儿和大于胎龄儿（LGA）的发生率高于新鲜 ET（14.2% vs. 10.3%；11.0% vs. 7.1%），调整后的率比分别为 1.45（95%CI，1.26-1.68；P<0.001）和 1.62（95%CI，1.37-1.91；P<0.001）。我们还观察到新鲜 ET 和 FET 分娩的胎盘中 PEG10mRNA 和蛋白表达水平明显不同，但两组间 L3MBTL1 无显著差异。新鲜 ET 可能影响胎盘印迹基因 PEG10 的表达，不利于胎盘着床和发育，导致 LBW 和 SGA 的发生率增加。
