Cai Yun-Xin, Xie Xiao-Yan, Zhou Qing-Qing, Huang Xiong, Xu Jin-Biao
College of Medicine, Jiaxing University Jiaxing 314001, China.
Zhongguo Zhong Yao Za Zhi. 2022 Jun;47(11):2994-2999. doi: 10.19540/j.cnki.cjcmm.20220307.202.
Silica gel, octadecyl-silica(ODS), Sephadex LH-20, and semi-preparative high performance liquid chromatography(HPLC) was performed to isolate nine cephalotaxine-type alkaloids from Cephalotaxus sinensis: 8-oxodeoxyharringtonine(1), 8-oxonordeoxyharringtonine(2), cephafortunine A(3), 8-oxocephalotaxine(4), deoxyharringtonine(5), acetylcephalotaxine(6), cephalotaxine(7), epicephalotaxine(8), and cephalotaxinone(9). Compounds 1 and 2 were identified for the first time and their structures were determined by high-resolution-electrospray ionization-mass spectrometry(HR-ESI-MS), nuclear magnetic resonance(NMR), and electronic circular dichroism(ECD). Compounds 1-3 and 5 significantly inhibited the transcription of nuclear factor kappa B(NF-κB), with the half-maximal inhibitory concentration(IC_(50)) of(3.91±0.70),(2.99±0.45),(7.84±0.51), and(1.46±0.17) μmol·L~(-1), respectively.
采用硅胶、十八烷基硅胶（ODS）、葡聚糖凝胶LH - 20以及半制备高效液相色谱（HPLC）从中国粗榧中分离出9种三尖杉碱型生物碱：8 - 氧代脱氧三尖杉酯碱（1）、8 - 氧代去甲脱氧三尖杉酯碱（2）、三尖福林碱A（3）、8 - 氧代三尖杉碱（4）、脱氧三尖杉酯碱（5）、乙酰三尖杉碱（6）、三尖杉碱（7）、表三尖杉碱（8）和三尖杉酮碱（9）。化合物1和2为首次鉴定，其结构通过高分辨电喷雾电离质谱（HR - ESI - MS）、核磁共振（NMR）和电子圆二色光谱（ECD）确定。化合物1 - 3和5显著抑制核因子κB（NF - κB）的转录，其半数抑制浓度（IC₅₀）分别为（3.91±0.70）、（2.99±0.45）、（7.84±0.51）和（1.46±0.17）μmol·L⁻¹。
