Wang Yuan, Zhou Jing, Yuan Ming-Ming, Zheng Xiao-Han, Zhang Lei
Chengdu University of Traditional Chinese Medicine Chengdu 611137, China Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China.
Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China.
Zhongguo Zhong Yao Za Zhi. 2022 Jun;47(11):3023-3028. doi: 10.19540/j.cnki.cjcmm.20220124.401.
This study aims to explore the protective effect of Forsythiae Fructus extract(FFE) against herpes simplex virus encephalitis(HSE) in mice. To be specific, life extension rate of mice, viral load in mouse brain, levels of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), and interferon-α(IFN-α), and nitric oxide(NO) content in mouse brain were determined. Mice were classified into normal group, model group, acyclovir(ACV) group, and high-dose, medium-dose, and low-dose(100, 50, 25 mg·kg~(-1), respectively) FFE groups. HSE was induced in mice in corresponding groups. Then, the life extension rate was compared among groups. Viral load in brain was detected by real-time fluorescent quantitative PCR, the changes of TNF-α, IL-1β, and IFN-α in brain by enzyme-linked immunosorbent assay(ELISA), NO content in brain with nitrate reduction method, and pathological changes by hematoxylin-eosin(HE) staining. The result showed that the life extension rate in the high-dose, medium-dose, and low-dose FFE groups was 27.93%, 19.94%, and 10.66%, respectively, and the difference between the high-dose group and the model group was statistically significant(P<0.05). FFE decreased the viral load in brains of HSE mice. The levels of TNF-α, IL-1β, and IFN-α in ACV group and high-dose and medium-dose FFE groups were lower than those in the model group(P<0.01,P<0.05), and NO content in the three FFE groups was lower than that in the model group(P<0.01). In conclusion, FFE can improve the survival rate of HSE mice, reduce the load of herpes simplex virus type Ⅰ(HSV-1) in the brains of HSE mice, decrease the levels of inflammatory factors and NO content, and alleviate inflammation and pathological damage, thereby protecting the central nervous system.
本研究旨在探讨连翘提取物（FFE）对小鼠单纯疱疹病毒性脑炎（HSE）的保护作用。具体而言，测定了小鼠的生命延长率、小鼠脑内病毒载量、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和干扰素-α（IFN-α）水平以及小鼠脑内一氧化氮（NO）含量。将小鼠分为正常组、模型组、阿昔洛韦（ACV）组以及高剂量、中剂量和低剂量（分别为100、50、25 mg·kg⁻¹）FFE组。对相应组的小鼠诱导HSE。然后，比较各组间的生命延长率。通过实时荧光定量PCR检测脑内病毒载量，采用酶联免疫吸附测定（ELISA）法检测脑内TNF-α、IL-1β和IFN-α的变化，用硝酸还原法检测脑内NO含量，并用苏木精-伊红（HE）染色观察病理变化。结果显示，高剂量、中剂量和低剂量FFE组的生命延长率分别为27.93%、19.94%和10.66%，高剂量组与模型组之间的差异具有统计学意义（P<0.05）。FFE降低了HSE小鼠脑内的病毒载量。ACV组以及高剂量和中剂量FFE组的TNF-α、IL-1β和IFN-α水平低于模型组（P<0.01，P<0.05），三个FFE组的NO含量低于模型组（P<0.01）。综上所述，FFE可提高HSE小鼠的生存率，降低HSE小鼠脑内Ⅰ型单纯疱疹病毒（HSV-1）载量，降低炎性因子水平和NO含量，减轻炎症和病理损伤，从而保护中枢神经系统。
