Cohen B J, Danon D, Roth G S
J Gerontol. 1987 May;42(3):295-301. doi: 10.1093/geronj/42.3.295.
The closure of bilateral, full-thickness cutaneous wounds made over the back with a sharp paper punch was measured with calipers and assessed histologically in C57BL/6J male mice for 10 days after wounding. Young (6 months) mice exhibited a significantly more rapid rate of wound closure and repair than did mature (15 months) or aged (26 or 27 months) mice. The repair rate in mature and aged mice did not differ. Young mice, injected subcutaneously at the wound sites with rabbit antimouse macrophage serum (RAMMS) 5 min before wounding and on days 1 and 3 after wounding, exhibited slow delayed closure of cutaneous wounds during days 1 to 4 after wounding, similar to that of untreated aged mice. The early closure rate of mice injected with normal rabbit serum or physiological saline was rapid, resembling that of untreated young mice. The results suggest that cutaneous wound repair in mice is another physiological phenomenon whose rate of change is age related, but not necessarily progressive to senescence. The results also imply that macrophage functional decline may contribute to the slowing of wound repair in middle-aged and aged mice compared to young mice.
用锋利的打孔器在C57BL/6J雄性小鼠背部制造双侧全层皮肤伤口，用卡尺测量伤口闭合情况，并在受伤后10天进行组织学评估。年轻（6个月）小鼠的伤口闭合和修复速度明显比成熟（15个月）或老年（26或27个月）小鼠快。成熟小鼠和老年小鼠的修复速度没有差异。在受伤前5分钟以及受伤后第1天和第3天，在伤口部位皮下注射兔抗小鼠巨噬细胞血清（RAMMS）的年轻小鼠，在受伤后第1至4天皮肤伤口出现缓慢的延迟闭合，类似于未处理的老年小鼠。注射正常兔血清或生理盐水的小鼠早期闭合速度很快，类似于未处理的年轻小鼠。结果表明，小鼠皮肤伤口修复是另一种生理现象，其变化速度与年龄有关，但不一定会逐渐衰老。结果还表明，与年轻小鼠相比，巨噬细胞功能下降可能导致中年和老年小鼠伤口修复减慢。
