Skjold S A, Quie P G, Fries L A, Barnham M, Cleary P P
J Infect Dis. 1987 Jun;155(6):1145-50. doi: 10.1093/infdis/155.6.1145.
A collection of Streptococcus zooepidemicus strains from human and animal infections was examined for DNA banding patterns after nuclease digestion and agarose gel electrophoresis. The large variety of DNA fingerprints found revealed the complexity of the species but showed that isolates from clusters of outbreaks had identical prints. The results confirmed the specificity of bacteriocin and bacteriophage typing of S. zooepidemicus; the technique also gave useful profiles on untypable strains. Strains with common bacteriocin and biotyping patterns from sporadic infections could be differentiated by their DNA fingerprints. In several outbreaks and incidents, more than one strain of S. zooepidemicus were encountered, and the importance of carefully interpreting typing data is stressed. Chromosomal DNA fingerprinting is a very efficient technique for demonstrating differences between strains of S. zooepidemicus, and its use is recommended for future epidemiological studies of this infectious agent.
对从人类和动物感染中分离出的兽疫链球菌菌株进行了核酸酶消化和琼脂糖凝胶电泳后，检测其DNA条带模式。所发现的大量DNA指纹图谱揭示了该物种的复杂性，但表明来自暴发集群的分离株具有相同的图谱。结果证实了兽疫链球菌细菌素和噬菌体分型的特异性；该技术也为无法分型的菌株提供了有用的图谱。散发性感染中具有共同细菌素和生物分型模式的菌株可通过其DNA指纹图谱进行区分。在几起暴发和事件中，遇到了不止一株兽疫链球菌，强调了仔细解读分型数据的重要性。染色体DNA指纹图谱是一种非常有效的技术，可用于证明兽疫链球菌菌株之间的差异，建议在今后对这种传染源的流行病学研究中使用。
