Renaud F, Freney J, Etienne J, Bes M, Brun Y, Barsotti O, Andre S, Fleurette J
Laboratoire de Bactériologie-Virologie, Centre National de référence des Staphylocoques, Faculté de Médecine Alexis Carrel, Lyon, France.
J Clin Microbiol. 1988 Sep;26(9):1729-34. doi: 10.1128/jcm.26.9.1729-1734.1988.
We compared the epidemiological markers of 13 Staphylococcus epidermidis strains isolated from an adult inpatient during a febrile episode and 23 S. epidermidis strains isolated during a presumptive outbreak of nosocomial infection in a neonatal ward. The total DNA restriction endonuclease analysis (REA) was processed along with the following conventional markers: biotyping, serotyping, phage typing, antibiotic susceptibility profiles, and plasmid profiles. The REA method was reproducible, giving stable results both in vitro and in vivo. For the hospitalized adult patient, the conventional markers of the 13 strains were concordant and the restriction profiles were identical. Five restriction groups were demonstrated during the course of the outbreak. Within two of the groups, the identities of all of the markers were used to verify whether all of the isolates belonged to the same cell clone. In a third group, combined analysis of the conventional markers and REA had to be used to demonstrate isolate similarity. On the other hand, in another group, none of the markers were similar; interpretation was not easy. An epidemiological study of S. epidermidis infections in hospitals must take into account all of the epidemiological markers: biotypes, serotypes, phage types, antibiograms, plasmid profiles, and REA.
我们比较了从一名成年住院患者发热期分离出的13株表皮葡萄球菌菌株和从新生儿病房疑似医院感染暴发期间分离出的23株表皮葡萄球菌菌株的流行病学标志物。进行了总DNA限制性内切酶分析（REA），并结合以下传统标志物：生物分型、血清分型、噬菌体分型、抗生素敏感性谱和质粒谱。REA方法具有可重复性，在体外和体内均能给出稳定的结果。对于住院成年患者，13株菌株的传统标志物一致，限制性图谱相同。在暴发过程中显示出五个限制性组。在其中两个组内，利用所有标志物的一致性来验证所有分离株是否属于同一细胞克隆。在第三组中，必须结合传统标志物和REA进行分析以证明分离株的相似性。另一方面，在另一组中，没有一个标志物相似，解读并不容易。医院中表皮葡萄球菌感染的流行病学研究必须考虑所有流行病学标志物：生物型、血清型、噬菌体型、抗菌谱、质粒谱和REA。
