Grattard F, Etienne J, Pozzetto B, Tardy F, Gaudin O G, Fleurette J
Laboratoire de Bactériologie-Virologie, Faculté de Médecine J. Lisfranc, Saint-Etienne, France.
J Clin Microbiol. 1993 Apr;31(4):812-8. doi: 10.1128/jcm.31.4.812-818.1993.
The molecular characteristics of 31 unrelated strains of Staphylococcus schleiferi isolated from 13 hospitals between 1973 and 1991 were determined by ribosomal DNA fingerprinting by using a digoxigenin-labeled DNA probe, genomic DNA restriction patterns, and plasmid profiles. Only six strains harbored one or two plasmids. DNA restriction analysis, which was carried out with five endonucleases (EcoRI, HindIII, PstI, PvuII, and ClaI), did not allow us to discriminate between isolates. Ribotyping with HindIII, ClaI, or EcoRI enzymes generated six, seven, and nine distinct patterns, respectively. With the combination ClaI-EcoRI, 13 ribotypes were obtained among the 31 strains, suggesting a relative heterogeneity within the species. Moreover, all strains shared two or three common bands, according to the endonuclease used, which were relatively specific for S. schleiferi in comparison with the ribosomal banding patterns described for other coagulase-negative staphylococci. These results illustrate that ribotyping can be used for the epidemiological investigation of S. schleiferi isolates and possibly for taxonomic analysis in this species.
1973年至1991年间，从13家医院分离出31株不相关的施氏葡萄球菌菌株，通过使用地高辛标记的DNA探针进行核糖体DNA指纹分析、基因组DNA限制性图谱分析和质粒图谱分析，确定了它们的分子特征。只有6株菌株含有一两个质粒。用5种核酸内切酶（EcoRI、HindIII、PstI、PvuII和ClaI）进行的DNA限制性分析，无法区分这些分离株。用HindIII、ClaI或EcoRI酶进行核糖体分型分别产生了6种、7种和9种不同的图谱。使用ClaI-EcoRI组合，在31株菌株中获得了13种核糖体分型，表明该物种内存在相对异质性。此外，根据所使用的核酸内切酶，所有菌株共有两三条共同条带，与其他凝固酶阴性葡萄球菌的核糖体条带模式相比，这些条带对施氏葡萄球菌具有相对特异性。这些结果表明，核糖体分型可用于施氏葡萄球菌分离株的流行病学调查，并可能用于该物种的分类分析。
