重组人β-防御素130通过破坏细胞膜抑制食源细菌的生长，并发挥抗炎活性。
Recombinant human β-defensin130 inhibited the growth of foodborne bacteria through membrane disruption and exerted anti-inflammatory activity.
作者信息
Dong Bin, Lin Yanjun, Su Zhiwei, Sun Chunlong, Wang Jun, Fu Shijun, Du Wen, Wu Tao
机构信息
Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, College of Biological and Environmental Engineering, Binzhou University, Binzhou, China.
Present Address: College of Biological and Environmental Engineering, Binzhou University, 391 Huanghe 5th Road, Binzhou City, 256603 Shandong Province China.
出版信息
Food Sci Biotechnol. 2022 Apr 20;31(7):893-904. doi: 10.1007/s10068-022-01087-y. eCollection 2022 Jul.
UNLABELLED
Foodborne pathogens causing food poisoning and infections are detrimental to human health, and the abuse of antibiotics induced severe antibiotic resistance in past decades. Thus, it is urgent to develop new antimicrobial agents. In the current study, human β-defensin 130 (hBD130), which is an antimicrobial peptide identified in human macrophages in 2017, was initially produced in . The purified hBD130 demonstrated broad bactericidal spectrum against foodborne pathogens through a membrane disruption, with concentrations ranging from 10 to 45 μg/mL. Moreover, hBD130 showed a low hemolytic effect and nearly no cytotoxicity to mammalian cells with a dosage of 400 μg/mL. In addition, the secretion amounts and mRNA levels of NO, IL-6, IL-1β, and TNF-α in LPS-induced mouse macrophage were significantly decreased with 1 mg/mL of hBD130. Taken together, these results showed that hBD130 is a promising antimicrobial agent to treat foodborne bacterial infections and inflammation.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-022-01087-y.
未标记
引起食物中毒和感染的食源性病原体对人类健康有害，在过去几十年中抗生素的滥用导致了严重的抗生素耐药性。因此，开发新型抗菌剂迫在眉睫。在当前研究中，人β-防御素130（hBD130）于2017年在人类巨噬细胞中被鉴定为一种抗菌肽，最初在……中产生。纯化后的hBD130通过破坏细胞膜对食源性病原体表现出广泛的杀菌谱，浓度范围为10至45μg/mL。此外，hBD130在剂量为400μg/mL时对哺乳动物细胞表现出低溶血效应且几乎无细胞毒性。另外，用1mg/mL的hBD130处理脂多糖诱导的小鼠巨噬细胞后，一氧化氮（NO）、白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的分泌量和mRNA水平显著降低。综上所述，这些结果表明hBD130是一种有前景的用于治疗食源性细菌感染和炎症的抗菌剂。
补充信息
在线版本包含可在10.1007/s10068-022-01087-y获取的补充材料。
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