Emirzeoglu Levent, Olmez Ozgur, Mustafayev Fatma Nihan Akkoc, Berber Ufuk, Yilmaz Ismail, Celik Serkan, Oven Bala Basak, Ozgun Mehmet Alpaslan
Department of Medical Oncology, University of Health Sciences, Sultan II. Abdulhamid Khan Educational and Research Hospital, 34660 Istanbul, Turkey.
Department of Pathology, University of Health Sciences, Sultan II. Abdulhamid Khan Educational and Research Hospital, 34660 Istanbul, Turkey.
Oncol Lett. 2022 May 26;24(1):226. doi: 10.3892/ol.2022.13347. eCollection 2022 Jul.
Aberrant expression of human leukocyte antigen G (HLA-G), an immunosuppressive molecule, has been observed in various cancer types. The exact mechanism of HLA-G expression is unclear. HLA-G expression is associated with low expression levels of microRNA (miR)-148a and miR-152. To the best of our knowledge, the prognostic value of the expression levels of miR-148a, miR-152 and HLA-G has not been investigated in colon cancer. The aim of the present study was to investigate the relationship between the presence of HLA-G molecules and the expression levels of miR-148 and miR-152 in colon cancer. In addition, the association of both HLA-G and miR expression with survival results and clinicopathological data was determined. An immunohistochemical method was used for detection of HLA-G expression in the tumor tissues and adjacent healthy tissues of 108 patients with colon cancer. Reverse transcription-quantitative PCR was used for miR analysis. HLA-G was expressed in 82 (75.9%) of the cancer samples, and there was no staining in normal tissues. HLA-G expression >20%, which was found in 41.7% (45/108) of the patients with colon cancer, was significantly positively associated with patient survival (P=0.039). Additionally, miR-148a levels were lower in the tumor tissues compared with the adjacent healthy tissues. No differences were found for miR-152 expression. In addition, mir-148a levels were found to be significantly lower (P=0.001) in HLA-G-positive tumor tissues than HLA-G-negative tumor tissues, but no such relationship was found for miR-152 levels. The presence of HLA-G expression was associated with poor survival outcomes. HLA-G is one of the prognostic factors in colon cancer, and decreased miR-148a expression in colon cancer tissues may be associated with HLA-G-mediated carcinogenesis.
人类白细胞抗原G（HLA - G）是一种免疫抑制分子，其异常表达已在多种癌症类型中被观察到。HLA - G表达的确切机制尚不清楚。HLA - G表达与微小RNA（miR）- 148a和miR - 152的低表达水平相关。据我们所知，miR - 148a、miR - 152和HLA - G表达水平在结肠癌中的预后价值尚未得到研究。本研究的目的是探讨结肠癌中HLA - G分子的存在与miR - 148和miR - 152表达水平之间的关系。此外，还确定了HLA - G和miR表达与生存结果及临床病理数据的关联。采用免疫组织化学方法检测108例结肠癌患者肿瘤组织及相邻健康组织中HLA - G的表达。采用逆转录定量PCR进行miR分析。82例（75.9%）癌症样本中表达HLA - G，正常组织无染色。41.7%（45/108）的结肠癌患者HLA - G表达>20%，与患者生存显著正相关（P = 0.039）。此外，肿瘤组织中miR - 148a水平低于相邻健康组织。miR - 152表达未发现差异。此外，发现HLA - G阳性肿瘤组织中mir - 148a水平显著低于HLA - G阴性肿瘤组织（P = 0.001），但miR - 152水平未发现这种关系。HLA - G表达的存在与不良生存结果相关。HLA - G是结肠癌的预后因素之一，结肠癌组织中miR - 148a表达降低可能与HLA - G介导的致癌作用有关。
