Wu Qikui, Chen Hong, Zhang Zihan, Chen Chen, Yu Fangyuan, Guy Robert D
Collaborative Innovation Centre of Sustainable Forestry in Southern China, College of Forest Science, Nanjing Forestry University, Nanjing, China.
State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow River, College of Forestry, Shandong Agricultural University, Tai'an, China.
Front Plant Sci. 2022 Jun 2;13:905633. doi: 10.3389/fpls.2022.905633. eCollection 2022.
has great potential as a biofuel feedstock source having industrial oilseeds with excellent fatty acids (FAs) composition and good fuel properties. Photosynthesis in the developing pericarp could affect the carbon distribution in kernel. During kernel development, more carbon sources are allocated to starch rather than lipid, when the pericarp photosynthesis is reduced by fruit shading treatment. After shading the fruits at 50 days after flowering (DAF), samples of shaded fruit (FSK) and controls (CK) were collected at 80 DAF and analyzed using the proteomic method. We identified 3,181 proteins, of which 277 were differentially expressed proteins, all downregulated in the FSK group. There were 56 proteins found involved in carbohydrate metabolism and lipid biosynthesis leading to oil accumulation with their iTRAQ ratios of FSK/CK ranging from 0.7123 to 1.1075. According to the qRT-PCR analyses, the key genes related to FA and triacylglycerol (TAG) biosynthesis were significantly downregulated between 60 and 90 DAF especially at 80 DAF, while the key genes involved in starch biosynthesis and FA desaturase had no significant difference between the two groups at 80 DAF. Fruit shading is a negative treatment for lipid accumulation but not starch accumulation by restraining enzymic protein expression involved in FA and TAG biosynthesis during kernel development.
作为一种生物燃料原料来源具有巨大潜力，其工业用油籽具有优异的脂肪酸（FAs）组成和良好的燃料特性。发育中的果皮光合作用会影响籽粒中的碳分配。在籽粒发育过程中，当通过果实遮光处理降低果皮光合作用时，更多的碳源被分配到淀粉而不是脂质中。在开花后50天（DAF）对果实进行遮光处理后，在80 DAF收集遮光果实（FSK）和对照（CK）的样本，并使用蛋白质组学方法进行分析。我们鉴定出3181种蛋白质，其中277种是差异表达蛋白质，在FSK组中均下调。发现有56种蛋白质参与碳水化合物代谢和脂质生物合成，导致油脂积累，其FSK/CK的iTRAQ比值在0.7123至1.1075之间。根据qRT-PCR分析，与脂肪酸和三酰甘油（TAG）生物合成相关的关键基因在60至90 DAF之间显著下调，尤其是在80 DAF时，而参与淀粉生物合成和脂肪酸去饱和酶的关键基因在80 DAF时两组之间没有显著差异。果实遮光通过抑制籽粒发育过程中参与脂肪酸和TAG生物合成的酶蛋白表达，对脂质积累是一种负面处理，但对淀粉积累不是。
