Enhanced Medicinal Ingredient Biosynthesis in Fisch. Under Different Conditions Based on the Transcriptome and Polymerase Chain Reaction Analysis.

The aim of this study was to evaluate the effect of () on the seedling growth and accumulation of medicinal ingredients of Fisch. () under control and salt stress conditions. Our results revealed the different effects of on the seedling growth and accumulation of medicinal ingredients particularly in different conditions based on the transcriptome and polymerase chain reaction (PCR) analysis. Under the control condition, significantly increased the expression level of the β, , , , , , and genes and liquiritigenin content. Under salt stress, significantly increased root length and lateral root number of seedlings, the expression level of , β, , , , , , and genes, and the contents of glycyrrhizic acid and glycyrrhetinic acid. Notably, the effect of on the seedling growth and the medicinal ingredient biosynthesis was different under control and salt stress conditions. Specifically, the effect of on the seedling growth under salt stress was greater than that under the control condition. Moreover, increased liquiritigenin content under the control condition, which is closely related to flavone and flavonol biosynthesis, while it increased the contents of glycyrrhizic acid and glycyrrhetinic acid under salt stress, which is closely related to phenylpropanoid biosynthesis, and the MVA pathway is also involved. All in all, endophytes could be used as a sustainable tool to develop effective bioinoculants to enhance the contents of medicinal ingredients in .