School of biology and pharmaceutical engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.
School of Pharmacy, South-Central University for Nationalities, Wuhan, 430074, People's Republic of China.
BMC Genomics. 2020 Sep 15;21(1):639. doi: 10.1186/s12864-020-07056-1.
Entada phaseoloides (L.) Merr. is an important traditional medicinal plant. The stem of Entada phaseoloides is popularly used as traditional medicine because of its significance in dispelling wind and dampness and remarkable anti-inflammatory activities. Triterpenoid saponins are the major bioactive compounds of Entada phaseoloides. However, genomic or transcriptomic technologies have not been used to study the triterpenoid saponin biosynthetic pathway in this plant.
We performed comparative transcriptome analysis of the root, stem, and leaf tissues of Entada phaseoloides with three independent biological replicates and obtained a total of 53.26 Gb clean data and 116,910 unigenes, with an average N50 length of 1218 bp. Putative functions could be annotated to 42,191 unigenes (36.1%) based on BLASTx searches against the Non-redundant, Uniprot, KEGG, Pfam, GO, KEGG and COG databases. Most of the unigenes related to triterpenoid saponin backbone biosynthesis were specifically upregulated in the stem. A total of 26 cytochrome P450 and 17 uridine diphosphate glycosyltransferase candidate genes related to triterpenoid saponin biosynthesis were identified. The differential expressions of selected genes were further verified by qPT-PCR.
The dataset reported here will facilitate the research about the functional genomics of triterpenoid saponin biosynthesis and genetic engineering of Entada phaseoloides.
含羞草科植物含羞草（L.）Merr. 是一种重要的传统药用植物。含羞草的茎因其祛风除湿、显著的抗炎活性而被广泛用作传统药物。三萜皂苷是含羞草的主要生物活性化合物。然而，基因组或转录组技术尚未用于研究该植物中的三萜皂苷生物合成途径。
我们对含羞草的根、茎和叶组织进行了三次独立的比较转录组分析，共获得了 53.26 Gb 的清洁数据和 116910 个 unigenes，平均 N50 长度为 1218bp。根据 BLASTx 对非冗余、Uniprot、KEGG、Pfam、GO、KEGG 和 COG 数据库的搜索，将 42191 个 unigenes（36.1%）推测出了功能。大多数与三萜皂苷骨架生物合成相关的 unigenes在茎中特异性地上调。总共鉴定出 26 个与三萜皂苷生物合成相关的细胞色素 P450 和 17 个尿苷二磷酸糖基转移酶候选基因。通过 qPT-PCR 进一步验证了所选基因的差异表达。
本报告的数据集中包含的信息有助于含羞草三萜皂苷生物合成的功能基因组学和遗传工程研究。
