Ye Haolong, Wang Jinqiu, Wang Ning, Wu Di, Li Hanmei, Geng Fang
Institute for Egg Science and Technology, School of Food and Biological Engineering, Chengdu University, 2025 Chengluo Avenue, Chengdu, 610106, China.
College of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
Curr Res Food Sci. 2022 Jun 9;5:964-972. doi: 10.1016/j.crfs.2022.05.013. eCollection 2022.
Egg-yolk low-density lipoprotein (LDL) has a natural liposome structure. Using ultrasound-assisted pH-shifting (pH 12), a naturally safe and stable aqueous solution of vitamin D (VD) was constructed employing LDL as the carrier. Images from electron microscopy showed that pH-shifting remodeled LDL molecules, resulting in a dramatic reduction in particle size (∼50%) and an increase in specific surface area, which reduced the turbidity (27.7%) and provided new interfaces for VD loading. Fluorescence analyses showed that the binding of VD to LDL under pH-shifting was strong, involved quenching, and the binding constant was 6.19 × 10 M. Thermogravimetric analysis and Fourier transform-infrared spectroscopy showed that pH-shifting hydrolyzed the esters in LDL to fatty acid salts, and the maximum weight loss of LDL occurred from 381.9 °C to 457.0 °C. Ultrasonic treatment enhanced the binding of LDL and VD (binding constant increased to 2.56 × 10 M), reduced the particle size, and increased the ζpotential of the complex between LDL and VD, thereby resulting in the improvement of solution stability and storage stability of VD. Ultrasound-assisted pH-shifting could remodel LDL to construct a stable aqueous solution of VD, which showed the potential of LDL as a carrier for lipid-soluble components.
蛋黄低密度脂蛋白（LDL）具有天然脂质体结构。利用超声辅助pH转变（pH 12），以LDL为载体构建了天然安全稳定的维生素D（VD）水溶液。电子显微镜图像显示，pH转变重塑了LDL分子，导致粒径显著减小（约50%），比表面积增加，降低了浊度（27.7%），并为VD负载提供了新的界面。荧光分析表明，在pH转变条件下VD与LDL的结合很强，涉及猝灭，结合常数为6.19×10 M。热重分析和傅里叶变换红外光谱表明，pH转变将LDL中的酯水解为脂肪酸盐，LDL的最大失重发生在381.9℃至457.0℃之间。超声处理增强了LDL与VD的结合（结合常数增加到2.56×10 M），减小了粒径，并增加了LDL与VD复合物的ζ电位，从而提高了VD溶液的稳定性和储存稳定性。超声辅助pH转变可以重塑LDL以构建稳定的VD水溶液，这显示了LDL作为脂溶性成分载体的潜力。
