Seth Caous Josefin, Svensson Malchau Karin, Petzold Max, Fridell Ylva, Malchau Henrik, Ahlstrom Linda, Grant Peter, Erichsen Andersson Annette
Department of Methodology, Textiles and Medical Technology, RISE Research Institutes of Sweden, Gothenburg, Sweden.
Department of Orthopedics, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Sweden.
Infect Prev Pract. 2022 May 26;4(3):100222. doi: 10.1016/j.infpip.2022.100222. eCollection 2022 Sep.
Airborne bacteria present in the operating room may be a cause of surgical site infection, either contaminating the surgical wound directly, or indirectly via e.g. surgical instruments. The aim of this study was to evaluate if instrument and assistant tables equipped with local unidirectional airflow reduce bacterial contamination of the instrument area to ultra clean levels, during orthopedic implant surgery in an operating room with displacement ventilation.
Local airflow units of instrument and assistant tables were either active or inactive. Colony forming units were sampled intraoperatively from the air above the instruments and from instrument dummies. A minimum of three air samples and two-three samples from instrument dummies were taken during each surgery. Samples were incubated on agar for total aerobic bacterial count. The mean air and instrument contamination during each surgery was calculated and used to analyze the difference in contamination depending on use of local airflow or not. All procedures were performed in the same OR.
188 air and 124 instrument samples were collected during 48 orthopedic implant procedures. Analysis showed that local unidirectional airflow above the surgical instruments significantly reduced the bacterial count in the air above assistant table (<0.001) and instrument table (=0.002), as well as on the instrument dummies from the assistant table (=0.001).
Instrumentation tables equipped with local unidirectional airflow protect the surgical instruments from bacterial contamination during orthopedic implant surgery and may therefore reduce the risk of indirect wound contamination.
手术室中存在的空气传播细菌可能是手术部位感染的一个原因,要么直接污染手术伤口,要么通过例如手术器械间接污染。本研究的目的是评估在采用置换通风的手术室进行骨科植入手术期间,配备局部单向气流的器械台和辅助台是否能将器械区域的细菌污染降低到超洁净水平。
器械台和辅助台的局部气流装置处于开启或关闭状态。术中从器械上方的空气和器械模型上采集菌落形成单位。每次手术期间至少采集三个空气样本和两到三个器械模型样本。样本在琼脂上培养以进行需氧细菌总数计数。计算每次手术期间的平均空气和器械污染情况,并用于分析根据局部气流使用与否造成的污染差异。所有操作均在同一手术室进行。
在48例骨科植入手术过程中收集了188个空气样本和124个器械样本。分析表明,手术器械上方的局部单向气流显著降低了辅助台上方空气中(<0.001)、器械台上方空气中(=0.002)以及辅助台器械模型上(=0.001)的细菌数量。
配备局部单向气流的器械台在骨科植入手术期间可保护手术器械免受细菌污染,因此可能降低间接伤口污染的风险。