Costa Susana P, Cunha Alexandra P, Freitas Paulo P, Carvalho Carla M
Centre of Biological Engineering, University of Minho, Braga, Portugal.
LABBELS -Associate Laboratory, Braga/Guimarães, Portugal.
Front Microbiol. 2022 Jun 1;13:871855. doi: 10.3389/fmicb.2022.871855. eCollection 2022.
is a problematic pathogen that causes life-threatening diseases, being a frequent causative agent of several nosocomial infections such as urinary tract and bloodstream infections. Proper and rapid bacterial identification is critical for allowing prompt and targeted antimicrobial therapy. (Bacterio)phage receptor-binding proteins (RBPs) display high specificity for bacterial surface epitopes and, therefore, are particularly attractive as biorecognition elements, potentially conferring high sensitivity and specificity in bacterial detection. In this study, we elucidated, for the first time, the potential of a recombinant RBP (Gp17) to recognize at different viability states, such as viable but not culturable cells, which are not detected by conventional techniques. Moreover, by using a diagnostic method in which we combined magnetic and spectrofluorimetric approaches, we demonstrated the ability of Gp17 to specifically detect in various human specimens (e.g., whole blood, feces, urine, and saliva) in about 1.5 h, without requiring complex sample processing.
是一种引发危及生命疾病的致病性病原菌，是多种医院感染（如尿路感染和血流感染）的常见病原体。准确快速的细菌鉴定对于及时进行有针对性的抗菌治疗至关重要。（细菌）噬菌体受体结合蛋白（RBPs）对细菌表面表位具有高度特异性，因此作为生物识别元件特别有吸引力，有可能在细菌检测中提供高灵敏度和特异性。在本研究中，我们首次阐明了重组RBP（Gp17）在不同生存状态下识别的潜力，例如传统技术无法检测到的活但不可培养细胞。此外，通过使用一种结合磁性和光谱荧光方法的诊断方法，我们证明了Gp17能够在约1.5小时内特异性检测各种人体标本（如全血、粪便、尿液和唾液）中的，而无需复杂的样品处理。
