Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan.
Laboratory of Microbiology, Department of Paraclinic, Faculty of Veterinary Sciences, Kabul University, Jamal Mina, Kabul 1006, Afghanistan.
Avian Dis. 2022 Jun;66(2):176-180. doi: 10.1637/aviandiseases-D-22-00002. Epub 2022 Jun 15.
Newcastle disease viruses (NDVs) in Afghanistan were isolated from three chicken farms and identified using a hemagglutination test and reverse transcription-polymerase chain reaction assay. Three isolates from each farm were sequenced to characterize the part of their fusion protein gene around the cleavage site. The characteristics of the fusion protein genes of the three isolates shown by phylogenic analysis indicated that the isolates were velogenic, belonged to the class II subgenotype VII 1.1, and were closely related to an identified Chinese NDV isolate. To our knowledge, this is the first time that NDV isolates from Afghanistan have been partially sequenced.
阿富汗的新城疫病毒（NDV）从三个养鸡场分离出来，并通过血凝试验和反转录聚合酶链反应（RT-PCR）鉴定。从每个农场的三个分离株中进行测序，以对其融合蛋白基因在裂解位点附近的部分进行特征分析。系统进化分析显示，三个分离株的融合蛋白基因特征表明它们为强毒力，属于 II 类亚基因型 VII 1.1，与已鉴定的中国 NDV 分离株密切相关。据我们所知，这是首次对阿富汗的 NDV 分离株进行部分测序。
