Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China.
Sci Total Environ. 2022 Oct 1;841:156790. doi: 10.1016/j.scitotenv.2022.156790. Epub 2022 Jun 17.
Landfill cover soils (LCS) play important roles in mitigating methane emissions from landfills. Anaerobic oxidation of methane (AOM) has been demonstrated as a potential methane removal process in aquatic ecosystems. However, whether AOM could occur in LCS is largely unknown. Here, microcosm incubations with CH were applied to track the potential activities of AOM and quantitative PCR was used to identify the responsible microorganisms. AOM was found to be active in the bottom and middle layers of LCS. In the bottom layer, sulfate-AOM was the most active process, mainly dominated by ANME archaea (without ANME-2d). Meanwhile, in the middle layer, nitrate and nitrite were the major electron acceptors involved in AOM with high abundances of ANME-2d archaea and NC10 bacteria. Our results implied a spatial segregation of methane oxidizing microbes in LCS and might be helpful for future control of methane emissions by the enhancement of AOM.
垃圾填埋覆盖土壤（LCS）在减少垃圾填埋场甲烷排放方面发挥着重要作用。甲烷的厌氧氧化（AOM）已被证明是水生生态系统中一种潜在的甲烷去除过程。然而，AOM 是否能在 LCS 中发生还知之甚少。在这里，应用 CH 的微宇宙培养来跟踪 AOM 的潜在活性，并用定量 PCR 来鉴定负责的微生物。结果发现 AOM 在 LCS 的底层和中层是活跃的。在底层，硫酸盐-AOM 是最活跃的过程，主要由 ANME 古菌（没有 ANME-2d）主导。同时，在中层，硝酸盐和亚硝酸盐是参与 AOM 的主要电子受体，含有大量的 ANME-2d 古菌和 NC10 细菌。我们的结果表明 LCS 中甲烷氧化微生物存在空间隔离，这可能有助于通过增强 AOM 来控制甲烷排放。
