School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China.
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; Key Laboratory of Biometallurgy, Ministry of Education, Changsha 410083, China.
Bioresour Technol. 2022 Sep;359:127487. doi: 10.1016/j.biortech.2022.127487. Epub 2022 Jun 17.
This work explored the microbial mechanisms for the improvement of composting efficiency driven by thermotolerant lignin-degrading bacterium Aneurinibacillus sp. LD3 (LD3). Results showed that LD3 inoculant prolonged the thermophilic period by 4 days, improved the final content of humic acid, total phosphorus (TP), nitrogen, potassium and seed germination index. Inoculating LD3 enhanced the relative abundance of thermotolerant and phosphate-solubilizing microbes including the phyla of Proteobacteria, Bacteroidota, Firmicutes, and Actinobacteriota, and the genus of Bacillus, Thermoactinomyces, and Pseudomonas. Metabolic function analysis showed that sequences involved in carbohydrate and amino acid metabolism were boosted, while sequences associated with human disease were reduced after inoculating LD3. Spearman correlation analysis revealed that Aneurinibacillus has a significant positive correlation with temperature, TP, Bacillus, and Thermoactinomyces. This study provides useful information for understanding the microbial mechanisms of LD3 promoting composting efficiency, and reveals the tremendous potential of LD3 in the resource utilization of organic solid wastes.
本研究探讨了耐热木质素降解菌 Aneurinibacillus sp. LD3（LD3）促进堆肥效率提高的微生物机制。结果表明，LD3 菌剂延长了高温期 4 天，提高了腐殖酸、总磷（TP）、氮、钾和种子发芽指数的最终含量。接种 LD3 增强了耐热和溶磷微生物的相对丰度，包括变形菌门、拟杆菌门、厚壁菌门和放线菌门，以及芽孢杆菌属、嗜热放线菌属和假单胞菌属。代谢功能分析表明，接种 LD3 后，碳水化合物和氨基酸代谢相关序列增加，而与人类疾病相关的序列减少。Spearman 相关分析表明，Aneurinibacillus 与温度、TP、芽孢杆菌和嗜热放线菌呈显著正相关。本研究为理解 LD3 促进堆肥效率的微生物机制提供了有用信息，揭示了 LD3 在有机固体废物资源利用方面的巨大潜力。
