Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; Engineering Laboratory for Yellow River Delta Modern Agriculture, Chinese Academy of Sciences, Beijing 100101, China.
Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; Engineering Laboratory for Yellow River Delta Modern Agriculture, Chinese Academy of Sciences, Beijing 100101, China.
Bioresour Technol. 2022 Sep;359:127489. doi: 10.1016/j.biortech.2022.127489. Epub 2022 Jun 17.
Antibiotic resistance genes (ARGs) in manure aerobic composting are a potential environmental pollutant. Therefore, reducing the abundance of ARGs is crucial. The effects of adding three-dimensional printed bulking agents (3DBAs) on ARGs in aerobic composting of swine manure were investigated in this study. Compared with the control group, 3DBAs with different addition dosages can greatest reduce the total ARGs by 5.98%, tetracycline resistance genes by 14.02%, macrolide resistance genes by 9.65%, and sulfonamide resistance genes by 20.59%. By further combining physicochemical parameters, host microorganisms, and mobile genetic elements (MGEs) for analysis, it was found that oxygen concentration was vital for ARGs reduction, and 3DBAs with regular porosity and uniform size indirectly regulate the activity of host microorganisms and MGEs abundance by changing the oxygen consumption, finally reducing vertical or horizontal ARGs transfer risks. Overall, 3DBAs addition is an effective strategy to reduce the abundance of ARGs in aerobic composting.
在粪便好氧堆肥中，抗生素抗性基因（ARGs）是一种潜在的环境污染物。因此，降低 ARGs 的丰度至关重要。本研究考察了添加三维打印膨胀剂（3DBA）对猪粪好氧堆肥中 ARGs 的影响。与对照组相比，不同添加剂量的 3DBA 可最大程度地将总 ARGs 减少 5.98%，将四环素抗性基因减少 14.02%，将大环内酯类抗性基因减少 9.65%，将磺胺类抗性基因减少 20.59%。通过进一步结合理化参数、宿主微生物和可移动遗传元件（MGEs）进行分析，发现氧浓度对 ARGs 减少至关重要，而具有规则孔隙率和均匀尺寸的 3DBA 可通过改变耗氧量间接调节宿主微生物和 MGEs 丰度的活性，最终降低垂直或水平的 ARGs 转移风险。总体而言，添加 3DBA 是降低好氧堆肥中 ARGs 丰度的有效策略。
