Key Laboratory of Agricultural Microbiology of Heilongjiang Province Science and Technology Department, Northeast Agricultural University, Harbin, 150030, China; College of Life Science, Northeast Agricultural University, Harbin, 150030, China.
Beijing Tongzhou Agriculture Products Quality Inspection & Testing Center, Beijing, 101149, China.
Environ Pollut. 2019 Jul;250:166-174. doi: 10.1016/j.envpol.2019.04.037. Epub 2019 Apr 8.
Mobile genetic elements (MGEs) play critical roles in transferring antibiotic resistance genes (ARGs) among different microorganisms in the environment. This study aimed to explore the fate of MGEs during chicken manure (CM) and bovine manure (BM) composting to assess horizontal transfer risks of ARGs. The results showed that the removal efficiency of MGEs during CM composting was significantly higher than that during BM composting, because the potential host bacteria of MGEs were eliminated largely during CM composting. Meanwhile, these potential host bacterial communities are significantly influenced by pH, NH, NO and total N, which can be used to regulate host bacterial communities to remove MGEs during composting. Projection pursuit regression further confirmed that composting can effectively reduce the horizontal transfer risk of ARGs, especially for CM composting. These results identified the critical roles of host bacterial communities in MGEs removal during composting of different animal manures.
移动遗传元件（MGEs）在环境中不同微生物之间转移抗生素抗性基因（ARGs）方面发挥着关键作用。本研究旨在探讨 MGEs 在鸡粪（CM）和牛粪（BM）堆肥过程中的命运，以评估 ARGs 的水平转移风险。结果表明，在 CM 堆肥过程中 MGEs 的去除效率明显高于在 BM 堆肥过程中的去除效率，因为在 CM 堆肥过程中 MGEs 的潜在宿主细菌被大量消除。同时，这些潜在的宿主细菌群落受 pH、NH、NO 和总 N 的显著影响，这些因素可用于调节堆肥过程中的宿主细菌群落以去除 MGEs。投影寻踪回归进一步证实，堆肥可以有效地降低 ARGs 的水平转移风险，尤其是对于 CM 堆肥。这些结果确定了在不同动物粪便堆肥过程中宿主细菌群落在 MGEs 去除中的关键作用。
