Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning 530021, China.
Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation, Pharmaceutical College, Guangxi Medical University, Nanning 530021, China.
J Environ Sci (China). 2022 Jul;117:91-104. doi: 10.1016/j.jes.2022.02.048. Epub 2022 Mar 17.
Iodoacetic acid (IAA) is an unregulated disinfection byproduct in drinking water and has been shown to exert cytotoxicity, genotoxicity, tumorigenicity, and reproductive and developmental toxicity. However, the effects of IAA on gut microbiota and its metabolism are still unknown, especially the association between gut microbiota and the metabolism and toxicity of IAA. In this study, female and male Sprague-Dawley rats were exposed to IAA at 0 and 16 mg/kg bw/day daily for 8 weeks by oral gavage. Results of 16S rRNA gene sequencing showed that IAA could alter the diversity, relative abundance and function of gut microbiota in female and male rats. IAA also increased the abundance of genes related to steroid hormone biosynthesis in the gut microbiota of male rats. Moreover, metabolomics profiling revealed that IAA could significantly disturb 6 and 13 metabolites in the feces of female and male rats, respectively. In female rats, the level of androstanediol increased in the IAA treatment group. These results were consistent with our previous findings, where IAA was identified as an androgen disruptor. Additionally, the perturbed gut microbiota and altered metabolites were correlated with each other. The results of this study indicated that IAA could disturb gut microbiota and its metabolism. These changes in gut microbiota and its metabolism were associated with the reproductive and developmental toxicity of IAA.
碘乙酸（IAA）是饮用水中不受监管的消毒副产物，已被证明具有细胞毒性、遗传毒性、致癌性、生殖毒性和发育毒性。然而，IAA 对肠道微生物群及其代谢的影响尚不清楚，特别是肠道微生物群与 IAA 的代谢和毒性之间的关联。在这项研究中，雌性和雄性 Sprague-Dawley 大鼠通过口服灌胃分别以 0 和 16mg/kg bw/天的剂量每天暴露于 IAA 8 周。16S rRNA 基因测序的结果表明，IAA 可以改变雌性和雄性大鼠肠道微生物群的多样性、相对丰度和功能。IAA 还增加了雄性大鼠肠道微生物群中与类固醇激素生物合成相关的基因的丰度。此外，代谢组学分析表明，IAA 可显著扰乱雌性和雄性大鼠粪便中的 6 和 13 种代谢物。在雌性大鼠中，IAA 处理组的雄烷二醇水平升高。这些结果与我们之前的研究结果一致，即 IAA 被鉴定为一种雄激素干扰物。此外，失调的肠道微生物群和改变的代谢物相互关联。本研究结果表明，IAA 可以扰乱肠道微生物群及其代谢。这些肠道微生物群及其代谢的变化与 IAA 的生殖毒性和发育毒性有关。
