Cooper J R, Pearce L B, Benishin C G
J Physiol (Paris). 1986;81(4):266-9.
A factor (Substance B) has been isolated from brain which reverses the presynaptically-modulated inhibition of evoked ACh release from both guinea-pig myenteric plexus-longitudinal muscle synaptosomes and the intact strip. Inhibitory modulating agents whose activity is reversed by Substance B include oxotremorine, 2-chloroadenosine, clonidine, and morphine. In addition to brain, Substance B is also present in heart and ileum but not in liver or kidney. As determined by Biogel P2 chromatography, this factor appears to have a molecular weight of around 700. It is not destroyed by preincubation with periodate, amylase, adenosine deaminase, pronase, trypsin, phospholipase C or carboxypeptidase Y.
已从大脑中分离出一种因子（物质B），它可逆转豚鼠肠肌丛-纵肌突触体和完整肠条中由突触前调制的诱发乙酰胆碱释放的抑制作用。其活性可被物质B逆转的抑制性调节剂包括氧化震颤素、2-氯腺苷、可乐定和吗啡。除大脑外，物质B也存在于心脏和回肠中，但不存在于肝脏或肾脏中。通过Biogel P2色谱法测定，该因子的分子量似乎约为700。用高碘酸盐、淀粉酶、腺苷脱氨酶、链霉蛋白酶、胰蛋白酶、磷脂酶C或羧肽酶Y预孵育不会破坏它。
