Li Xu, Wang Gonglei, Chen Jiabo, Zhou Xin, Liu Yu
College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China; Innovation Center for Postgraduate Education in Municipal Engineering of Shanxi Province, Taiyuan, 030024, China.
College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China; Innovation Center for Postgraduate Education in Municipal Engineering of Shanxi Province, Taiyuan, 030024, China.
Chemosphere. 2022 Oct;305:135409. doi: 10.1016/j.chemosphere.2022.135409. Epub 2022 Jun 18.
One-stage anammox-based autotrophic nitrogen removal technology has attracted increasing interest to sustainable biological nitrogen removal for future wastewater treatment. However, its application in mainstream municipal wastewater treatment is still challenging due to low nitrogen and high organics of raw wastewater. Herein, a novel Simultaneous Carbon Oxidation, partial Comammox, Denitratation and Anammox (SCOCDA) was firstly developed in a single sequencing batch biofilm reactor operated at a dissolved oxygen concentration of ∼0.5 mg/L for treating synthetic municipal wastewater (50 mg/L NH-N and 100-250 mg/L COD). The long-term operation showed that almost complete COD and nitrogen removal performance could be achieved at a carbon/nitrogen ratio (COD/NH-N) of 3-5 with the corresponding effluent total nitrogen (TN)＜5 mg/L. Microbial community and amoA-targeting amplicon sequencing analysis further verified that comammox Nitrospira spp., denitrifier Thauera and other aerobic/facultative heterotrophs could work synergistically with anammox bacteria, Candidatus Kuenenia. Moreover, nitrogen metabolic and inorganic carbon fixation pathways through the interaction between comammox and anammox were also revealed with the aid of Kyoto Encyclopedia of Genes and Genomes (KEGG). Lastly, potential application of proposed SCOCDA process was illustrated. This research sheds new light on advanced nitrogen removal towards limit of technology via the synergy of comammox and anammox.
基于厌氧氨氧化的单级自养脱氮技术在未来污水处理的可持续生物脱氮方面越来越受到关注。然而，由于原废水氮含量低且有机物含量高，其在城市污水处理主流工艺中的应用仍具有挑战性。在此，首次在溶解氧浓度约为0.5mg/L的单级序批式生物膜反应器中开发了一种新型的同步碳氧化、部分全程自养脱氮、反硝化和厌氧氨氧化（SCOCDA）工艺，用于处理合成城市污水（50mg/L NH-N和100-250mg/L COD）。长期运行表明，在碳氮比（COD/NH-N）为3-5时，几乎可以实现完全的COD和氮去除性能，相应的出水总氮（TN）＜5mg/L。微生物群落和靶向amoA的扩增子测序分析进一步证实，全程自养脱氮菌硝化螺菌属、反硝化菌陶厄氏菌属和其他好氧/兼性异养菌可以与厌氧氨氧化细菌“库氏假单胞菌”协同工作。此外，借助京都基因与基因组百科全书（KEGG）还揭示了通过全程自养脱氮菌和厌氧氨氧化菌之间的相互作用产生的氮代谢和无机碳固定途径。最后，阐述了所提出的SCOCDA工艺的潜在应用。本研究通过全程自养脱氮菌和厌氧氨氧化菌的协同作用，为先进的脱氮技术极限研究提供了新的思路。
