Harrison L M, Kenny N, Niswender G D
J Reprod Fertil. 1987 Mar;79(2):539-48. doi: 10.1530/jrf.0.0790539.
Corpora lutea were collected from sheep on Days 6, 10, and 15 of the oestrous cycle and Day 25 of pregnancy and dissociated into single cell suspensions. Purified preparations of large and small luteal cells were prepared by elutriation on all days except Day 6. Basal progesterone production by large cells was 6-8-fold higher than by small cells (36-65 vs 6-9 fg/cell/min). Oxytocin secretion was maximal on Day 6 (1.0 fg/cell/min) and declined thereafter. The number of receptors for LH increased between Day 6 and Day 10 and the two cell types had an equal number of receptors on Days 10 and 15 (19,000-23,000). Large cells on Day 25 of pregnancy had fewer receptors (12,000) than did small cells (26,000). Progesterone secretion by small luteal cells from all days examined was stimulated by LH (0.01-1000 ng/ml) in a dose-dependent manner; maximum sensitivity to LH occurred on Day 10. Despite the presence of receptors for LH on large cells, LH failed to stimulate progesterone production. Basal production of progesterone by large and small cells, and the response of small cells to LH, was not influenced by day examined. Re-combinations of large and small cells from Day 10 synergized to increase progesterone secretion. Prostaglandin E-2 (0.1-1000 ng/ml) did not stimulate progesterone secretion by large or small cells.
在发情周期的第6天、第10天和第15天以及妊娠第25天从绵羊体内收集黄体，并将其解离成单细胞悬液。除第6天外，在所有其他日子通过淘洗法制备大、小黄体细胞的纯化制剂。大细胞的基础孕酮分泌量比小细胞高6 - 8倍（36 - 65 fg/细胞/分钟对6 - 9 fg/细胞/分钟）。催产素分泌在第6天最高（1.0 fg/细胞/分钟），此后下降。促黄体生成素（LH）受体数量在第6天到第10天增加，在第10天和第15天两种细胞类型的受体数量相等（19,000 - 23,000个）。妊娠第25天的大细胞受体数量（12,000个）比小细胞（26,000个）少。在所检测的所有日子里，小黄体细胞的孕酮分泌受到LH（0.01 - 1000 ng/ml）的剂量依赖性刺激；对LH的最大敏感性出现在第10天。尽管大细胞上存在LH受体，但LH未能刺激孕酮生成。大、小细胞的基础孕酮分泌以及小细胞对LH的反应不受检测日期的影响。来自第10天的大、小细胞重组可协同增加孕酮分泌。前列腺素E - 2（0.1 - 1000 ng/ml）未刺激大、小细胞的孕酮分泌。
