Institute of Plant Production and Plant Breeding, Grass and Forage Science/Organic Agriculture, Kiel University (CAU), 24118, Kiel, Germany.
Institute of Animal Nutrition and Physiology, Kiel University (CAU), 24118, Kiel, Germany.
Sci Rep. 2022 Jun 21;12(1):10454. doi: 10.1038/s41598-022-14424-2.
An in vitro Hohenheim gas test was conducted to analyze the fermentation end-products from 17 cultivars of eight polyphenol containing forage species. The polyphenol composition and proanthocyanidin (PA) structural features of all the cultivars were analyzed with UPLC-MS/MS in leaves of vegetative or generative plants. The samples were incubated with and without polyethylene glycol (PEG, a tannin-binding agent) to separate the tannin-effect on methane (CH ml/200 mg DM) production from that of forage quality. Sulla and big trefoil, two particularly PA rich species, were found to have the highest CH reduction potential of up to 47% when compared to the samples without PEG. However, concomitant reduction in gas production (GP, ml/200 mg DM) of up to 44% was also observed. An increase in both GP and CH production under PEG treatments, confirms the role of tannins in CH reduction. Moreover, PA structural features and concentration were found to be an important source of variation for CH production from PA containing species. Despite having low polyphenol concentrations, chicory and plantain were found to reduce CH production without reducing GP. Additionally, interspecies variability was found to be higher than intraspecies variability, and these results were consistent across growth stages, indicating the findings' representativeness.
进行了体外 Hohenheim 气体测试，以分析来自 8 个多酚含量丰富的饲料物种的 17 个品种的发酵终产物。使用 UPLC-MS/MS 分析了所有品种叶片中多酚的组成和原花青素（PA）结构特征，无论是在营养生长还是生殖生长阶段的植物。将样品用和不用聚乙二醇（PEG，一种单宁结合剂）进行孵育，以分离单宁对甲烷（CH ml/200 mg DM）产生的影响和饲料质量的影响。与没有 PEG 的样品相比，Sulla 和大三叶草这两种特别富含 PA 的物种，其 CH 还原潜力最高可达 47%。然而，同时也观察到气体产生（GP，ml/200 mg DM）减少了 44%。PEG 处理下 GP 和 CH 产生的增加，证实了单宁在 CH 还原中的作用。此外，PA 的结构特征和浓度是 PA 含量丰富的物种产生 CH 的重要变异来源。尽管多酚浓度较低，但菊苣和车前草被发现可以在不降低 GP 的情况下减少 CH 的产生。此外，种间变异性高于种内变异性，并且这些结果在不同的生长阶段都一致，表明结果具有代表性。
