利用进化的分裂 RNA 聚合酶进行农业化学控制基因表达。
Agrochemical control of gene expression using evolved split RNA polymerase.
机构信息
Department of Neurophysiology and Neuropharmacology, Institute of Special Environmental Medicine and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China.
Duke Kunshan University, Kunshan, China.
出版信息
PeerJ. 2022 Jun 16;10:e13619. doi: 10.7717/peerj.13619. eCollection 2022.
Chemically-inducible gene expression systems are valuable tools for rational control of gene expression both for basic research and biotechnology. However, most chemical inducers are confined to certain groups of organisms. Therefore, dissecting interactions between different organisms could be challenging using existing chemically-inducible systems. We engineered a mandipropamid-induced gene expression system (Mandi-T7) based on evolved split T7 RNAP system. As a proof-of-principle, we induced GFP expression in cells grown inside plant tissue.
化学诱导型基因表达系统是一种用于理性控制基因表达的有价值的工具，无论是对于基础研究还是生物技术都是如此。然而，大多数化学诱导剂仅限于某些生物群体。因此，使用现有的化学诱导型系统来解析不同生物之间的相互作用可能具有挑战性。我们基于进化的分裂 T7 RNA 聚合酶系统，设计了一种 mandipropamid 诱导型基因表达系统（Mandi-T7）。作为原理验证，我们在植物组织内部生长的 细胞中诱导 GFP 表达。
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