Department of Otorhinolaryngology, Toho University School of Medicine, Tokyo, Japan.
Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan.
Acta Otolaryngol. 2022 Jun;142(6):537-541. doi: 10.1080/00016489.2022.2086292. Epub 2022 Jun 22.
External auditory canal (EAC) squamous cell carcinoma (SCC) is a rare disease, and the survival rate is low in the advanced stages. It has been reported that miR-34a expression is low in many cancers and acts as a tumor suppressor, but its function in EACSCC has not yet been reported.
To analyze the miR-34a expression levels in EACSCC specimens using hybridization (ISH).
We performed microRNA ISH for miR-34a detection and immunohistochemical analysis of p53 and Ki67 in the EACSCC and otitis externa (OE) specimens.
miR-34a was expressed in the basal and suprabasal layers in the OE epidermis. The pronounced expression of miR-34a was observed in the two cases of T2 (Stage II). In the one case of T3 (Stage III), it was almost the same as that of the OE. On the other hand, the expression levels of miR-34a in the one case of T3 (Stage IV) and two cases of T4 (Stage IV) were apparently reduced.
We demonstrated that the expression level of miR-34a was higher in early-stage EACSCC and lower in advanced-stage EACSCC.
The expression level of miR-34a may predict a prognosis in patients with advanced-stage EACSCC.
外耳道鳞状细胞癌（EACSCC）是一种罕见疾病，晚期生存率较低。已有报道称，许多癌症中 miR-34a 的表达水平较低，其作为一种肿瘤抑制因子发挥作用，但 miR-34a 在 EACSCC 中的功能尚未见报道。
采用杂交（ISH）技术分析 EACSCC 标本中 miR-34a 的表达水平。
我们对 EACSCC 和外耳道炎（OE）标本进行了 miR-34a 的 miRNA ISH 检测和 p53、Ki67 的免疫组织化学分析。
miR-34a 在 OE 表皮的基底细胞层和基底上层表达。在 T2（II 期）的两例中，miR-34a 的表达明显增强。在 T3（III 期）的一例中，与 OE 相似。另一方面，T3（IV 期）和 T4（IV 期）的两例中 miR-34a 的表达水平明显降低。
我们证明 miR-34a 的表达水平在 EACSCC 的早期阶段较高，在晚期阶段较低。
miR-34a 的表达水平可能预测晚期 EACSCC 患者的预后。
