Maldiney Thomas, Chassot Jean-Marie, Boccara Claude, Blot Mathieu, Piroth Lionel, Charles Pierre-Emmanuel, Garcia-Hermoso Dea, Lanternier Fanny, Dalle Frédéric, Sautour Marc
Department of Intensive Care Medicine, William Morey General Hospital, Chalon-sur-Saône, France; Lipness team, INSERM Research Center LNC-UMR1231, University of Burgundy, Dijon, France.
Institut Langevin, ESPCI Paris, PSL University, CNRS, 75005 Paris, France.
J Mycol Med. 2022 Nov;32(4):101303. doi: 10.1016/j.mycmed.2022.101303. Epub 2022 Jun 11.
Histopathology and microscopic examination of infected tissue are the gold standards to prove the diagnosis of invasive fungal infection (IFI). Yet, they suffer from essential limitations that hamper rapid diagnosis and require the future development of new imaging tools dedicated to fungal diagnostics. To this end, the present work introduces the first use of dynamic full-field optical coherence tomography (D-FF-OCT) for the visualization of microscopic filamentous fungi. Data collected from the observation of three different fungal species (Nannizzia gypsea, Aspergillus fumigatus and Rhizopus arrhizus) confirm the ability of D-FF-OCT to visualize not only the main structures of all selected fungal species (hyphae, spores, conidia, sporulating structures), but also the metabolic activity of the organisms, which could provide additional help in the future to better characterize the signature of each fungal structure. These results demonstrate how D-FF-OCT could serve as potential complementary tool for rapid diagnosis of IFI in both intensive and non-intensive care units.
感染组织的组织病理学和显微镜检查是确诊侵袭性真菌感染（IFI）的金标准。然而，它们存在一些根本性的局限性，阻碍了快速诊断，因此需要开发专门用于真菌诊断的新型成像工具。为此，本研究首次介绍了动态全场光学相干断层扫描（D-FF-OCT）用于可视化微观丝状真菌。对三种不同真菌物种（石膏样奈尼兹皮癣菌、烟曲霉和少根根霉）观察所收集的数据证实，D-FF-OCT不仅能够可视化所有选定真菌物种的主要结构（菌丝、孢子、分生孢子、产孢结构），还能观察到生物体的代谢活性，这在未来可能有助于更好地描述每种真菌结构的特征。这些结果表明，D-FF-OCT可作为重症监护病房和非重症监护病房快速诊断IFI的潜在补充工具。
