Azzollini Salvatore, Monfort Tual, Thouvenin Olivier, Grieve Kate
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012 Paris, France.
CHNO des Quinze-Vingts, INSERM-DGOS CIC 1423, 28 rue de Charenton, F-75012 Paris, France.
Biomed Opt Express. 2023 Jun 14;14(7):3362-3379. doi: 10.1364/BOE.488929. eCollection 2023 Jul 1.
Label-free live optical imaging of dynamic cellular and subcellular features has been made possible in recent years thanks to the advances made in optical imaging techniques, including dynamic optical coherence tomography (D-OCT) methods. These techniques analyze the temporal fluctuations of an optical signal associated with the active movements of intracellular organelles to obtain an ensemble metric recapitulating the motility and metabolic state of cells. They hence enable visualization of cells within compact, static environments and evaluate their physiology. These emerging microscopies show promise, in particular for the three-dimensional evaluation of live tissue samples such as freshly excised biopsies and 3D cell cultures. In this review, we compare the various techniques used for dynamic OCT. We give an overview of the range of applications currently being explored and discuss the future outlook and opportunities for the field.
近年来,得益于光学成像技术的进步,包括动态光学相干断层扫描(D-OCT)方法,对动态细胞和亚细胞特征进行无标记的实时光学成像已成为可能。这些技术分析与细胞内细胞器的活跃运动相关的光信号的时间波动,以获得概括细胞运动性和代谢状态的总体指标。因此,它们能够在紧凑的静态环境中可视化细胞并评估其生理状态。这些新兴的显微镜技术显示出了前景,特别是对于新鲜切除的活检组织和3D细胞培养等活组织样本的三维评估。在这篇综述中,我们比较了用于动态OCT的各种技术。我们概述了目前正在探索的应用范围,并讨论了该领域的未来前景和机遇。