Suppr超能文献

用于细胞分析的动态光学相干断层扫描[特邀报告]

Dynamic optical coherence tomography for cell analysis [Invited].

作者信息

Azzollini Salvatore, Monfort Tual, Thouvenin Olivier, Grieve Kate

机构信息

Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012 Paris, France.

CHNO des Quinze-Vingts, INSERM-DGOS CIC 1423, 28 rue de Charenton, F-75012 Paris, France.

出版信息

Biomed Opt Express. 2023 Jun 14;14(7):3362-3379. doi: 10.1364/BOE.488929. eCollection 2023 Jul 1.

Abstract

Label-free live optical imaging of dynamic cellular and subcellular features has been made possible in recent years thanks to the advances made in optical imaging techniques, including dynamic optical coherence tomography (D-OCT) methods. These techniques analyze the temporal fluctuations of an optical signal associated with the active movements of intracellular organelles to obtain an ensemble metric recapitulating the motility and metabolic state of cells. They hence enable visualization of cells within compact, static environments and evaluate their physiology. These emerging microscopies show promise, in particular for the three-dimensional evaluation of live tissue samples such as freshly excised biopsies and 3D cell cultures. In this review, we compare the various techniques used for dynamic OCT. We give an overview of the range of applications currently being explored and discuss the future outlook and opportunities for the field.

摘要

近年来,得益于光学成像技术的进步,包括动态光学相干断层扫描(D-OCT)方法,对动态细胞和亚细胞特征进行无标记的实时光学成像已成为可能。这些技术分析与细胞内细胞器的活跃运动相关的光信号的时间波动,以获得概括细胞运动性和代谢状态的总体指标。因此,它们能够在紧凑的静态环境中可视化细胞并评估其生理状态。这些新兴的显微镜技术显示出了前景,特别是对于新鲜切除的活检组织和3D细胞培养等活组织样本的三维评估。在这篇综述中,我们比较了用于动态OCT的各种技术。我们概述了目前正在探索的应用范围,并讨论了该领域的未来前景和机遇。

相似文献

1
Dynamic optical coherence tomography for cell analysis [Invited].
Biomed Opt Express. 2023 Jun 14;14(7):3362-3379. doi: 10.1364/BOE.488929. eCollection 2023 Jul 1.
2
Methods and applications of full-field optical coherence tomography: a review.
J Biomed Opt. 2022 May;27(5). doi: 10.1117/1.JBO.27.5.050901.
4
Dynamic full-field optical coherence tomography for live-cell imaging and growth-phase monitoring in .
Front Cell Infect Microbiol. 2023 Jul 12;13:1183340. doi: 10.3389/fcimb.2023.1183340. eCollection 2023.
5
Dynamic contrast optical coherence tomography (DyC-OCT) for label-free live cell imaging.
Commun Biol. 2024 Mar 6;7(1):278. doi: 10.1038/s42003-024-05973-5.
7
Enhanced medical diagnosis for dOCTors: a perspective of optical coherence tomography.
J Biomed Opt. 2021 Oct;26(10). doi: 10.1117/1.JBO.26.10.100601.
8
Time-domain full-field optical coherence tomography (TD-FF-OCT) in ophthalmic imaging.
Ther Adv Chronic Dis. 2023 May 2;14:20406223231170146. doi: 10.1177/20406223231170146. eCollection 2023.
9
10
Three-dimensional, label-free cell viability measurements in tissue engineering scaffolds using optical coherence tomography.
J Biomed Mater Res A. 2023 Aug;111(8):1279-1291. doi: 10.1002/jbm.a.37528. Epub 2023 Mar 14.

引用本文的文献

1
A modular and reconfigurable microfluidic device for culturing spheroids under continuous perfusion.
APL Bioeng. 2025 Aug 13;9(3):036111. doi: 10.1063/5.0262536. eCollection 2025 Sep.
2
FDA and EMA-approved noninvasive imaging techniques for basal cell carcinoma subtyping: A systematic review.
JAAD Int. 2025 Jun 10;21:73-86. doi: 10.1016/j.jdin.2025.05.006. eCollection 2025 Aug.
3
Optical coherence tomography for early detection of crop infection.
Plant Methods. 2025 Jul 6;21(1):92. doi: 10.1186/s13007-025-01411-7.
4
Using Optical Coherence Tomography in Plant Biology Research: Review and Prospects.
Sensors (Basel). 2025 Apr 14;25(8):2467. doi: 10.3390/s25082467.
5
Optical coherence tomography for label-free detection and characterization of methicillin-resistant biofilms.
J Biomed Opt. 2025 Apr;30(4):046003. doi: 10.1117/1.JBO.30.4.046003. Epub 2025 Apr 1.
7
Imaging-Guided Microscale Photothermal Stereolithography Bioprinting.
Adv Sci (Weinh). 2025 May;12(18):e2500640. doi: 10.1002/advs.202500640. Epub 2025 Mar 20.
8
A Survey on Optical Coherence Tomography-Technology and Application.
Bioengineering (Basel). 2025 Jan 14;12(1):65. doi: 10.3390/bioengineering12010065.
9
zIncubascope: Long-term quantitative imaging of multi-cellular assemblies inside an incubator.
PLoS One. 2025 Jan 23;20(1):e0309035. doi: 10.1371/journal.pone.0309035. eCollection 2025.
10
Line-field dynamic optical coherence tomography platform for volumetric assessment of biological tissues.
Biomed Opt Express. 2024 Jun 7;15(7):4162-4175. doi: 10.1364/BOE.527797. eCollection 2024 Jul 1.

本文引用的文献

2
Interface self-referenced dynamic full-field optical coherence tomography.
Biomed Opt Express. 2023 Jun 21;14(7):3491-3505. doi: 10.1364/BOE.488663. eCollection 2023 Jul 1.
3
Label-free metabolic imaging of non-alcoholic-fatty-liver-disease (NAFLD) liver by volumetric dynamic optical coherence tomography.
Biomed Opt Express. 2022 Jun 30;13(7):4071-4086. doi: 10.1364/BOE.461433. eCollection 2022 Jul 1.
5
Dynamic microscopic optical coherence tomography to visualize the morphological and functional micro-anatomy of the airways.
Biomed Opt Express. 2022 May 5;13(6):3211-3223. doi: 10.1364/BOE.456104. eCollection 2022 Jun 1.
6
Dynamic full-field optical coherence tomography as complementary tool in fungal diagnostics.
J Mycol Med. 2022 Nov;32(4):101303. doi: 10.1016/j.mycmed.2022.101303. Epub 2022 Jun 11.
9
Three-dimensional dynamics optical coherence tomography for tumor spheroid evaluation.
Biomed Opt Express. 2021 Oct 12;12(11):6844-6863. doi: 10.1364/BOE.440444. eCollection 2021 Nov 1.
10
Quantitative evaluation of the dynamic activity of HeLa cells in different viability states using dynamic full-field optical coherence microscopy.
Biomed Opt Express. 2021 Sep 21;12(10):6431-6441. doi: 10.1364/BOE.436330. eCollection 2021 Oct 1.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验