Furr H C, McCormick D B
Int J Vitam Nutr Res. 1978;48(2):165-9.
A soil bacterium, Pseudomonas putida LP, can be grown on lipoate as sole source of carbon, sulfur, and energy. In addition to the previously identified catabolites (bisnorlipoate, tetranorlipoate, beta-hydroxybisnorlipoate, lipoate thiolsulfinate, and two bisnorlipoate thiolsulfinates) isolated from cultures of the organism grown on [1,6-14C[lipoate, a methyl ketone (1,2-dithiolane-3-butyl-3'-one) has now been isolated and identified. This catabolite was isolated by solvent extraction and hydrophobic gel filtration and characterized by chromatographic mobilities and spray reactions and by UV, IR, PMR, and mass spectrometries. The methyl ketone presumably arises by decarboxylation of the beta-keto acid formed during the beta-oxidation of lipoate to bisnorlipoate by the microorganism.
一种土壤细菌，恶臭假单胞菌LP，能够以硫辛酸作为唯一的碳、硫和能量来源进行生长。除了之前从在[1,6-¹⁴C]硫辛酸上生长的该微生物培养物中分离出的已鉴定的分解代谢产物（双降硫辛酸、四降硫辛酸、β-羟基双降硫辛酸、硫辛酸硫代亚磺酸盐以及两种双降硫辛酸硫代亚磺酸盐）外，现在已分离并鉴定出一种甲基酮（1,2-二硫戊环-3-丁基-3'-酮）。该分解代谢产物通过溶剂萃取和疏水凝胶过滤进行分离，并通过色谱迁移率、喷雾反应以及紫外、红外、核磁共振和质谱分析进行表征。推测该甲基酮是由微生物将硫辛酸β氧化为双降硫辛酸过程中形成的β-酮酸脱羧产生的。
