Shukla O P
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Microbios. 1989;59(238):47-63.
A Gram-negative, oxidase positive, polar flagellated rod, characterised as Pseudomonas stutzeri, has been isolated from sewage by enrichment culture on quinoline. The organism utilizes quinoline as the sole source of carbon, nitrogen and energy, and liberates UV absorbing and phenolic metabolites during its growth on quinoline. 2-Hydroxyquinoline, 2,8-dihydroxyquinoline, 8-hydroxycoumarin and 2,3-dihydroxyphenylpropionic acid have been isolated as the transformation products of quinoline by this bacterium. Quinoline, 2-hydroxyquinoline, and 8-hydroxycoumarin were rapidly oxidised by quinoline-adapted cells; 2,3-dihydroxyphenylpropionic acid oxidation was also demonstrated by Warburg respirometry but 2,8-dihydroxyquinoline was not oxidised. A pathway for quinoline catabolism by P. stutzeri and the probable mechanisms for formation of 8-hydroxycoumarin are suggested.
通过在喹啉上进行富集培养，从污水中分离出了一种革兰氏阴性、氧化酶阳性、具极生鞭毛的杆菌，鉴定为施氏假单胞菌。该微生物利用喹啉作为唯一的碳、氮和能量来源，并在其于喹啉上生长期间释放出吸收紫外线的和酚类代谢产物。2-羟基喹啉、2,8-二羟基喹啉、8-羟基香豆素和2,3-二羟基苯丙酸已被分离出来，作为该细菌对喹啉的转化产物。喹啉、2-羟基喹啉和8-羟基香豆素被适应喹啉的细胞迅速氧化；通过瓦氏呼吸测定法也证实了2,3-二羟基苯丙酸的氧化，但2,8-二羟基喹啉未被氧化。提出了施氏假单胞菌对喹啉的分解代谢途径以及8-羟基香豆素形成的可能机制。
