CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, Vila do Conde, Portugal.
Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal.
Methods Mol Biol. 2022;2505:191-202. doi: 10.1007/978-1-0716-2349-7_14.
Transcriptional regulation is a central piece of the highly valuable monoterpenoid indole alkaloid pathway of C. roseus , and the ultimate tool for its understanding and manipulation. Here, we describe the adaptation of the TARGET methodology to identify specific and genome-wide leaf targets of C. roseus candidate transcription factors (TFs).
转录调控是长春花中具有高价值的单萜吲哚生物碱途径的核心部分，也是理解和操纵该途径的最终工具。在这里，我们描述了 TARGET 方法的适应性，以鉴定长春花候选转录因子 (TF) 的特定和全基因组叶片靶标。
