从甲基组学到组织特异性的综合分析。

From Methylome to Integrative Analysis of Tissue Specificity.

机构信息

EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France.

Limagrain, Centre de Recherches de Chappes, Route d'Ennezat, Chappes, France.

出版信息

Methods Mol Biol. 2022;2505:223-240. doi: 10.1007/978-1-0716-2349-7_16.

DOI:10.1007/978-1-0716-2349-7_16

PMID:35732948

Abstract

DNA methylation is the most studied epigenetic mark in both plants and animals. The gold standard for assaying genome-wide DNA methylation at single-base resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal plant Catharanthus roseus.

摘要

DNA 甲基化是植物和动物中研究最多的表观遗传标记。在单碱基分辨率上检测全基因组 DNA 甲基化的金标准是全基因组亚硫酸氢盐测序（WGBS）。在这里，我们描述了一种改进的 WGBS 程序和原始的生物信息学工作流程，用于揭示药用植物长春花中与基因表达和次生代谢物积累相关的组织特异性甲基组变化。